文献类型：期刊文献

英文题名：CbuERF114, an ethylene responsive factor, regulates floral transition in Catalpa bungei

作者：Fei, Yue[1,2] Yao, Chengcheng[3] Hu, Jiwen[3] Lu, Nan[3] Li, Pengle[3] Liu, Bingyang[3] Wang, Zhi[4] Shi, Chaozhong[4] Wu, Chuangye[5] Li, Pan[5] Zhang, Miaomiao[3] Wang, Junhui[3]

第一作者：Fei, Yue

通信作者：Zhang, MM[1];Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[4]Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China;[5]Wenxian Forestry Sci Res Inst, Jiaozuo 454850, Peoples R China

年份：2025

卷号：227

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-105008962871);WOS:【SCI-EXPANDED(收录号:WOS:001520005200001)】；

基金：This work was supported by the National Key Research and Development Program of China, China (2021YFD2200202) , the National Natural Science Foundation of China, China (32371912) , and the Chinese Academy of Forestry Sciences through the Special Funds for Central Public-Interest Scientific Institution Public Welfare Projects, China (CAFYBB2020SY005) . We thank the Wenxian Forestry Science Research Institute for providing the plant materials. We thank Dr. Yuan Cao from the State Key Laboratory of the Chinese Academy of Forestry for her excellent technical assistance with the morphological investigation (TEM/fluorescence observation) .

语种：英文

外文关键词：AP2/ERF; Catalpa bungei; Regulation of flowering time; CbuERF114; Floral transition; Flower quantity

摘要：Understanding the flowering of woody plants is crucial for controlling flowering time and shortening breeding cycles, yet the mechanisms are largely unknown. APETALA2/ethylene response factor (AP2/ERF) transcription factors are essential in floral transition and development. We report an early-flowering (EF) variety of Catalpa bungei that blooms in its planting year, whereas normal-flowering (NF) requires 5-7 years. This study identified 150 CbuAP2/ERF genes in the C. bungei genome, categorized into 4 subfamilies and 17 groups. Expression analysis showed AP2/ERF genes mainly activated during the vegetative to mixed buds transition and predominantly expressed in the stem. To explore CbuERF roles in floral transition, six genes (CbuERF3B/060/071/RAP2-3/114/003-4) induced by floral induction were selected. CbuERF114 expression differed significantly during the reproductive transition between NF and EF. Subcellular localization and transcriptional activation analysis indicated that CbuERF114 is a nuclear protein with a C-terminal transcriptional activation domain. Over-expressing CbuERF114 in Arabidopsis (OE-CbuERF114) delayed bolting and flowering by about 4 days but extended the flowering period, increasing the maximum flowering growth rate by 54.25% and the number of flowers by 58.00%. CbuERF114 inhibited flowering onset but enhanced flowering rate and quantity. These findings advance our understanding of CbuAP2/ERFs in phylogeny, structure, expression, and function, highlighting their importance in the floral transition of woody plants.