文献类型：期刊文献

中文题名：PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in Populus alba×P.glandulosa

作者：Jinnan Wang[1,2] Houjun Zhou[1,3] Yanqiu Zhao[1,4] Cheng Jiang[1,4] Jihong Li[2] Fang Tang[1,5] Yingli Liu[1] Shutang Zhao[1,5] Jianjun Hu[1,5] Xueqin Song[1,5] MengZhu Lu[1,4,5]

第一作者：Jinnan Wang

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of the State ForestryAdministration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;[2]Key Laboratory of Agricultural Ecology and Environment,College of Forestry,Shandong Agricultural University,Tai'an 271018,China;[3]School of Agriculture,Ludong University,Yantai 264025,China;[4]State Key Laboratory of Subtropical Silviculture,School of Forestry and Biotechnology,Zhejiang Agriculture&Forestry University,Hangzhou 311300,China;[5]CoInnovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China

年份：2021

卷号：63

期号：10

起止页码：1683-1694

中文期刊名：植物学报：英文版

外文期刊名：Journal of Integrative Plant Biology

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：This work was supported by the National Natural ScienceFoundation of China(31570676);the National Key Programon Transgenic Research of China(2018ZX08020-002);the Basic Research Fund of Research Institute of Forest,ChineseAcademy of Forestry(RIF-2014-08);the National Key Re-search and Development Program of China(2016YFD0600103);the National Key Program on Transgenic Re-search of China(2018ZX08020-002);the Ten-thousandTalents Program of China for Meng-Zhu Lu.

语种：英文

中文关键词：GRF;GIF1;Populus;wood formation;XND1;xylem development

分类号：Q945.4

摘要：Growth-regulating factors(GRFs)are important regulators of plant development and growth,but their possible roles in xylem development in woody plants remain unclear.Here,we report that Populus alba×Papulus glandulosa PagGRF12a negatively regulates xylem development in poplar.PagGRF12a is expressed in vascular tissues.Compared to non-transgenic control plants,transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem width.Xylem NAC domain 1(XND1)encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants,indicating that PagGRF12a may regulate xylem development through PagXND1a.Transient transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates PagXND1a.In addition,PagGRF12a interacts with the GRF-Interacting Factor(GIF)PagGIF1b,and this interaction enhances the effects of PagGRF12a on PagXND1a.Our results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.