文献类型：期刊文献

英文题名：A Comprehensive Analysis of Transcriptomics and Metabolomics Reveals Key Genes Involved in Terpenes Biosynthesis Pathway of Litsea cubeba Under Light and Darkness Treatments

作者：Yang, Jiahui[1,2,3] Zhao, Yunxiao[1,3] Chen, Yicun[1,3] Wang, Yangdong[1,3] Gao, Ming[1,3]

第一作者：Yang, Jiahui

通信作者：Gao, M[1];Gao, M[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coll Forest, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2025

卷号：26

期号：7

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-105002329468);WOS:【SCI-EXPANDED(收录号:WOS:001463999600001)】；

基金：This work was supported by the National Key Research and Development Program of China (2022YFD2200601) and the Zhejiang Province Key Research and Development Program (2024C02009).

语种：英文

外文关键词：Litsea cubeba; terpenoid; biosynthetic pathway; expression patterns

摘要：Light is an important environmental regulator of plant secondary metabolism. Terpenoids, the most abundant secondary metabolites in plants, demonstrate a wide spectrum of biologically significant properties, encompassing antimicrobial, antioxidative, and analgesic activities. Litsea cubeba (Lour.) Pers., a core species within the Lauraceae family, exhibits notable pharmacological potential, including antimicrobial and antitumor effects. Here, we found that darkness treatment significantly suppressed terpenoid accumulation in L. cubeba fruits. To clarify the molecular mechanisms underlying the regulatory effect of light and darkness treatments on terpenoid biosynthesis, we conducted a comparative transcriptome profiling of L. cubeba fruits under light and darkness treatments. A total of 13,074 differentially expressed genes (DEGs) were identified among four sampling time points (L1-L2-L3-L4 vs. D1-D2-D3-D4). These genes were enriched in various pathways, with significant enrichment being observed in the terpenoid and other secondary metabolism pathways. Additionally, the enrichment of DEGs in L2 and D2 stages was further studied, and it was found that nine DEGs were significantly enriched in the monoterpene synthesis pathway. The weighted gene co-expression network analysis (WGCNA) showed that alcohol dehydrogenase (ADH), a key enzyme in terpenoid synthesis, had the same expression pattern as WRKY and NAC transcription factors, suggesting their involvement in the biosynthesis of terpenoids in L. cubeba. Expression profiling demonstrated that plastid-localized terpenoid pathway genes were markedly downregulated under darkness treatment. qRT-PCR validation of key genes (LcDXS3, LcHMGS1, LcMDS, and LcTPS19) confirmed the reliability of the transcriptome data, with LcDXS3 exhibiting pronounced declines in expression after 6 h (2.76-fold decrease) and 12 h (2.63-fold decrease) of darkness treatment. These findings provide novel insights into the photoregulatory mechanisms governing terpenoid metabolism in L. cubeba.