文献类型：期刊文献

英文题名：Genome sequence and population genomics provide insights into chromosomal evolution and phytochemical innovation of Hippophae rhamnoides

作者：Yu, Liyang[1,2,3] Diao, Songfeng[1,2,4] Zhang, Guoyun[1,2] Yu, Jigao[5] Zhang, Tong[1,2] Luo, Hongmei[6] Duan, Aiguo[1,2] Wang, Jinpeng[5] He, Caiyun[1,2] Zhang, Jianguo[1,2,3]

第一作者：Yu, Liyang

通信作者：He, CY[1];Zhang, JG[1];He, CY[2];Zhang, JG[2];Zhang, JG[3];Wang, JP[4]|[a0005b245645e47e67b16]何彩云;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Peoples R China;[4]Chinese Acad Forestry, Res Inst Nontimber Forestry, Key Lab Nontimber Forest Germplasm Enhancement &, Zhengzhou, Peoples R China;[5]North China Univ Sci & Technol, Sch Life Sci, Tangshan, Peoples R China;[6]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou, Peoples R China

年份：2022

卷号：20

期号：7

起止页码：1257-1273

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000788196600001)】；

基金：This work was supported by grants from Special Fund for Forest Scientific Research in the Public Welfare (201504103), the National Natural Science Foundation of China (U2003116) and the Fundamental Research Funds of CAF (CAFYBB2020SZ001-2). The genome sequencing, assembly, and annotation were performed with the help of Biomarker Technologies.

语种：英文

外文关键词：seabuckthorn assembly; paleopolyploidizations; karyotype reconstruction; fatty acid; ascorbic acid

摘要：Plants of the Elaeagnaceae family are widely used to treat various health disorders owing to their natural phytochemicals. Seabuckthorn (Hippophae rhamnoides L.) is an economically and ecologically important species within the family with richness of biologically and pharmacologically active substances. Here, we present a chromosome-level genome assembly of seabuckthorn (http://hipp.shengxin.ren/), the first genome sequence of Elaeagnaceae, which has a total length of 849.04 Mb with scaffold N50 of 69.52 Mb and 30 864 annotated genes. Two sequential tetraploidizations with one occurring similar to 36-41 million years ago (Mya) and the last similar to 24-27 Mya were inferred, resulting in expansion of genes related to ascorbate and aldarate metabolism, lipid biosynthesis, and fatty acid elongation. Comparative genomic analysis reconstructed the evolutionary trajectories of the seabuckthorn genome with the predicted ancestral genome of 14 proto-chromosomes. Comparative transcriptomic and metabonomic analyses identified some key genes contributing to high content of polyunsaturated fatty acids and ascorbic acid (AsA). Additionally, we generated and analysed 55 whole-genome sequences of diverse accessions, and identified 9.80 million genetic variants in the seabuckthorn germplasms. Intriguingly, genes in selective sweep regions identified through population genomic analysis appeared to contribute to the richness of AsA and fatty acid in seabuckthorn fruits, among which GalLDH, GMPase and ACC, TER were the potentially major-effect causative genes controlling AsA and fatty acid content of the fruit, respectively. Our research offers novel insights into the molecular basis underlying phytochemical innovation of seabuckthorn, and provides valuable resources for exploring the evolution of the Elaeagnaceae family and molecular breeding.