文献类型：期刊文献

中文题名：油橄榄品种表型和SSR标记的多样性及聚类分析

英文题名：Diversity and Cluster Analysis on Phenotypic Traits and SSR of Olive Cultivars

作者：秦倩[1] 王楠楠[1] 李金花[1] 苏光灿[2]

第一作者：秦倩

机构：[1]林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所;[2]凉山州中泽新技术开发有限责任公司

年份：2016

卷号：29

期号：5

起止页码：676-681

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：中央级公益性科研院所基本科研业务费专项资助"油橄榄主栽品种授粉习性及杂交的研究"(CAFYBB2014MA001)

语种：中文

中文关键词：油橄榄;表型性状;SSR荧光标记;遗传多样性;聚类分析

外文关键词：Olea europaea L. , phenotypic data, SSR, genetic diversity, clustering analysis

分类号：S565.7

摘要：[目的]利用表型性状和分子标记,对我国油橄榄品种进行鉴定和遗传多样性研究,有利于种质资源保存和利用,对了解油橄榄品种的组成结构及未来的引种和育种工作都具有重要意义。[方法]以甘肃省陇南市17个油橄榄品种为研究材料,利用表型上15个数量性状、18个质量性状和8个SSR荧光标记分别进行多样性和聚类分析。[结果]各性状在品种间存在显著差异,数量性状和质量性状的表型多样性指数分别介于1.579 2.089和0.3621.091,8个SSR位点共检测到51个等位基因变异,平均每个位点等位基因数为6.375个,利用表型性状和SSR标记可以将17个品种完全区分开。[结论]17个油橄榄品种具有较丰富的表型和遗传多样性,基于表型数量性状、质量性状和SSR标记的聚类分析结果,判别品种间遗传关系的结果有一致的,也存在一定差异。对于表型性状相似的品种,需结合SSR标记进行品种鉴定。
[ Objective ] With genetic markers and phenotypic data to identify olive cuhivars and evaluation their ge- netic diversity. [ Method ] Taking 11 olive cultivars from Longnan, Gansu Province, as samples, the genetic diversity analysis and cluster analysis were conducted with 15 quantitative traits, 18 quality phenotypic traits and 8 SSR markers. [ Result ] For all the phenotypic traits, significant variability was found among the cuhivars. The phenotypic diversity index of quantitative traits ranged from 1. 579 to 2. 088 as those of quality traits ranged from 0. 362 to 1.091. A total of 51 alleles were detected for 8 SSR markers, while the number of alleles per locus reached an average of 5.9. With the phenotypic traits and SSR markers, 17 cuhivars can be completely identified and distinguished. [ Conclusion ] The result of genetic distance analysis based on phenotypic traits and SSR markers showed similar genetic relationships between cuhivars with somewhat differences between them. The SSR markers should be combined to identify cultivars with similar phenotypic traits.