文献类型：期刊文献

英文题名：Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription Factor JERF36 Gene in Two Different Environments

作者：Zhang, Weixi[1,2] Wang, Yanbo[1,2,3] Zhang, Tengqian[1,2] Zhang, Jing[1,2] Shen, Le[1,2] Zhang, Bingyu[1,2] Ding, Changjun[1,2] Su, Xiaohua[1,2,4]

第一作者：张伟溪;Zhang, Weixi

通信作者：Ding, CJ[1];Su, XH[1];Ding, CJ[2];Su, XH[2];Su, XH[3]

机构：[1]Res Inst Forestry, Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[3]Nanchang Inst Technol, Nanchang, Peoples R China;[4]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Peoples R China

年份：2022

卷号：10

外文期刊名：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000816981900001)】；

基金：The financial support of this study was from the National Natural Science Foundation of China (Grant No. 317700598), the Basic Research Fund of RIF (Grant No. CAFYBB2020SZ002) and the National Major Project of GMO New Species Cultivation (Grant No. 2018ZX08020002).

语种：英文

外文关键词：transgenic poplar; stress resistance; transcriptome analysis; differentially expressed genes; environmental effect

摘要：During the last several decades, a number of transgenic or genetically modified tree varieties with enhanced characteristics and new traits have been produced. These trees have become associated with generally unsubstantiated concerns over health and environmental safety. We conducted transcriptome sequencing of transgenic Populus alba x P. berolinensis expressing the transcription factor JERF36 gene (ABJ01) and the non-transgenic progenitor line (9#) to compare the transcriptional changes in the apical buds. We found that 0.77% and 1.31% of the total expressed genes were significant differentially expressed in ABJ01 at the Daqing and Qiqihar sites, respectively. Among them, 30%-50% of the DEGs contained cis-elements recognized by JERF36. Approximately 5% of the total number of expressed genes showed significant differential expression between Daqing and Qiqihar in both ABJ01 and 9#. 10 DEGs resulting from foreign gene introduction, 394 DEGs that resulted solely from the environmental differences, and 47 DEGs that resulted from the combination of foreign gene introduction and the environment were identified. The number of DEGs resulting from environmental factors was significantly greater than that resulting from foreign gene introduction, and the combined effect of the environmental effects with foreign gene introduction was significantly greater than resulting from the introduction of JERF36 alone. GO and KEGG annotation showed that the DEGs mainly participate in the photosynthesis, oxidative phosphorylation, plant hormone signaling, ribosome, endocytosis, and plant-pathogen interaction pathways, which play important roles in the responses to biotic and abiotic stresses ins plant. To enhance its adaptability to salt-alkali stress, the transgenic poplar line may regulate the expression of genes that participate in the photosynthesis, oxidative phosphorylation, MAPK, and plant hormone signaling pathways. The crosstalk between biotic and abiotic stress responses by plant hormones may improve the ability of both transgenic and non-transgenic poplars to defend against pathogens. The results of our study provide a basis for further studies on the molecular mechanisms behind improved stress resistance and the unexpected effects of transgenic gene expression in poplars, which will be significant for improving the biosafety evaluation of transgenic trees and accelerating the breeding of new varieties of forest trees resistant to environmental stresses.