文献类型：期刊文献

英文题名：A Homogenous Fluorescence Immunoassay Based on Dna-Grafted Labeling Strategy of Magnetic Microspheres for Multiple Neonicotinoids Analysis

作者：Zou, Rubing[1] Yang, Peiyu[1] Chen, Peitong[1] Wen, Zexin[1] Wang, Zhenyu[1] Wang, Yao[2] Liu, Yihua[3] Zhu, Guonian[1] Guo, Yirong[1]

第一作者：Zou, Rubing

机构：[1] Institute of Pesticide and Environmental Toxicology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, 310058, China; [2] School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China; [3] Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240174966)

语种：英文

外文关键词：Amides - Antibodies - Cost effectiveness - DNA - Fluorescence - Grafting (chemical) - Metabolites - Suspensions (fluids)

摘要：Given the detrimental impact on non-target pollinators, there is a high demand for monitoring multiple neonicotinoid residues in the environment. In this study, we proposed a novel physical-biological coupled multiplexing system (PBCMS) to achieve a homogeneous and high-performance indirect competitive suspension array technology (ic-SAT) for multiple neonicotinoids monitoring. First, hapten-decorated microspheres (HDMs) were prepared by ligating the streptavidin-modified microspheres with the biotinylated hapten, according to a novel molecularly-defined single-stranded DNA (ssDNA)-grafted labeling approach. Taking thiamethoxam (THX) and imidacloprid (IMI) as models, the binding properties of two anti-neonicotinoid antibodies were investigated by establishing homogeneous monoplex ic-SAT assays. Subsequently, two sized HDMs (2.8 and 5.0 μm) with corresponding haptens of THX and IMI were implemented to fabricate a dualplex ic-SAT assay, while they were distinguished via a facile diameter-resolved decoding process on flow cytometer. Excitingly, the screening capacity of dualplex ic-SAT was significantly upgraded by employing antibodies with distinctive binding profiles. It enables the simultaneous monitoring of 7 representative neonicotinoid insecticides (THX, IMI, acetamiprid, clothianidin, thiacloprid, nitenpyram and imidaclothiz) and 4 major metabolites (6-Cl-PMNI, N-desmethyl-acetamiprid, N-desmethyl-thiamethoxam and thiacloprid-amide) in a single test sample, with IC50 values ranging from 0.02 to 572.7 ng/mL. Furthermore, the fluorescence dualplex ic-SAT assay was applied to honey, pollen and bee specimens, demonstrating satisfactory recovery rates (74.1%~106.3%) and nice agreement with LC-MS/MS analysis. In summary, the PBCMS offers an easy-to-implement, robust and cost-effective way for establishing user-defined ic-SAT, providing a powerful analytical tool to simultaneously monitor multiple neonicotinoid residues in environmental samples. ? 2024, The Authors. All rights reserved.