文献类型：期刊文献

英文题名：De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer's properties

作者：Guo, Qianqian[1,2] Ma, Xiaojun[3,4] Wei, Shugen[5] Qiu, Deyou[6] Wilson, Iain W.[7] Wu, Peng[2] Tang, Qi[8] Liu, Lijun[1] Dong, Shoukun[1] Zu, Wei[1]

第一作者：Guo, Qianqian

通信作者：Ma, XJ[1]

机构：[1]Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China;[2]Qiqihar Univ, Coll Life Sci Agr & Forest, Qiqihar 161006, Peoples R China;[3]Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China;[4]Chinese Acad Med Sci, Inst Med Plant Dev, Yunnan Branch, Jinghong 530023, Peoples R China;[5]Guangxi Med Bot Garden, Nanning 530023, Peoples R China;[6]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[7]CSIRO Plant Ind, Canberra, ACT 2001, Australia;[8]Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China

年份：2014

卷号：15

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000340646500001)】；

基金：This work was supported by the central project of significant increase, decrease and support (Grant No. 2060302) and Guangxi National Science Foundation of China (grant No. 2012GXNSFBA053118).

语种：英文

外文关键词：Transcriptome; DGE; Uncaria rhynchophylla; Rhynchophylline; Isorhynchophylline; Terpene indole alkaloid biosynthesis

摘要：Background: The major medicinal alkaloids isolated from Uncaria rhynchophylla (gouteng in chinese) capsules are rhynchophylline (RIN) and isorhynchophylline (IRN). Extracts containing these terpene indole alkaloids (TIAs) can inhibit the formation and destabilize preformed fibrils of amyloid beta protein (a pathological marker of Alzheimer's disease), and have been shown to improve the cognitive function of mice with Alzheimer-like symptoms. The biosynthetic pathways of RIN and IRN are largely unknown. Results: In this study, RNA-sequencing of pooled Uncaria capsules RNA samples taken at three developmental stages that accumulate different amount of RIN and IRN was performed. More than 50 million high-quality reads from a cDNA library were generated and de novo assembled. Sequences for all of the known enzymes involved in TIAs synthesis were identified. Additionally, 193 cytochrome P450 (CYP450), 280 methyltransferase and 144 isomerase genes were identified, that are potential candidates for enzymes involved in RIN and IRN synthesis. Digital gene expression profile (DGE) analysis was performed on the three capsule developmental stages, and based on genes possessing expression profiles consistent with RIN and IRN levels; four CYP450s, three methyltransferases and three isomerases were identified as the candidates most likely to be involved in the later steps of RIN and IRN biosynthesis. Conclusion: A combination of de novo transcriptome assembly and DGE analysis was shown to be a powerful method for identifying genes encoding enzymes potentially involved in the biosynthesis of important secondary metabolites in a non-model plant. The transcriptome data from this study provides an important resource for understanding the formation of major bioactive constituents in the capsule extract from Uncaria, and provides information that may aid in metabolic engineering to increase yields of these important alkaloids.