文献类型：期刊文献

英文题名：Functional divergence of Populus MYB158 and MYB189 gene pair created by whole genome duplication

作者：Jiang, Peng-Fei[1,2] Xu, Hui[1,2] Guan, Chao-Nan[3,4] Wang, Xiao-Xia[3,4] Wu, Ai-Min[5] Liu, Yan-Jing[3] Zeng, Qing-Yin[3]

第一作者：Jiang, Peng-Fei

通信作者：Zeng, QY[1]

机构：[1]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[5]South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Peoples R China

年份：2022

卷号：60

期号：1

起止页码：169-185

外文期刊名：JOURNAL OF SYSTEMATICS AND EVOLUTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000537464500001)】；

基金：This study was supported by the National Science Fund for Distinguished Young Scholars (31425006) and the Chinese Academy of Forestry (CAFYBB2018ZX001).

语种：英文

外文关键词：duplicate gene; functional divergence; MYB transcription factor; regulatory networks; secondary cell wall biosynthesis; whole genome duplication

摘要：Whole genome duplication (WGD) provides new genetic material for genome evolution. After a WGD event, some duplicates are lost, while other duplicates still persist and evolve diverse functions. A particular challenge is to understand how this diversity arises. This study identified two WGD-derived duplicates, MYB158 and MYB189, from Populus tomentosa. Populus MYB158 and MYB189 had expression divergence. Populus tomentosa overexpressing MYB158 or MYB189 had similar phenotypes: creep growth, decreased width of xylem and secondary cell wall thickness. Compared to wild-type, neither myb158 mutant nor myb158 myb189 double mutant showed obvious phenotypic variation in P. tomentosa. Although MYB158 and MYB189 proteins could repress the same structural genes involved in lignin, cellulose, and xylan biosynthesis, the two proteins had their own specific regulatory targets. Populus MYB158 could act as the upstream regulator of secondary cell wall NAC master switch and directly represses the expression of the SND1-B2 gene. Taken together, Populus MYB158 and MYB189 have retained similar functions in negatively regulating secondary cell wall biosynthesis, but have evolved partially distinct functions in direct regulation of NAC master switch, with MYB158 playing a more crucial role. Our findings provide new insights into the evolutionary and functional divergence of WGD-derived duplicate genes.