文献类型：期刊文献

中文题名：Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar(Populus spp.)

英文题名：Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar(Populus spp.)

作者：Wenxu Zhu[1,2] Yanguang Chu[1,2] Changjun Ding[1,2] Qinjun Huang[1,2] Bingyu Zhang[1,2] Weixi Zhang[1,2] Xiaohua Su[1,2]

第一作者：Wenxu Zhu

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry, Chinese Academy of Forestry,Beijing 100091, China;[2]Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China

年份：2016

卷号：27

期号：4

起止页码：939-947

中文期刊名：林业研究：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2015_2016】；

基金：financially supported by the National High-Tech Research and Development Program of China,863Program(Grant No.2011AA100201);the National Forestry Public Welfare Research Project of China(Grant No.201004004)

语种：英文

中文关键词：转基因杨树;根际土壤;细菌群落;微生物群落;细菌多样性;评估;群落结构;非转基因

外文关键词：rhizosphere communities Bacteria sequencing poplar planted taxonomic representing genera microbial

分类号：S792.11;S791.22

摘要：Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified(GM) plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines(D520 and D521), one non-genetically modified(nonGM) line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups(with [1 % of all sequences) across the samples. 241 of 551 genera(representing a ratio of 97.33 %) were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units(OTUs) were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.
Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified（GM） plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines（D520 and D521）, one non-genetically modified（nonGM） line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups（with [1 % of all sequences） across the samples. 241 of 551 genera（representing a ratio of 97.33 %） were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units（OTUs） were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.