文献类型：期刊文献

英文题名：Integration of small RNAs, degradome and transcriptome sequencing in hyperaccumulator Sedum alfredii uncovers a complex regulatory network and provides insights into cadmium phytoremediation

作者：Han, Xiaojiao[1,2] Yin, Hengfu[2] Song, Xixi[1,2] Zhang, Yunxing[1,2] Liu, Mingying[1,2] Sang, Jiang[1,2] Jiang, Jing[1,2] Li, Jihong[3] Zhuo, Renying[1,2]

第一作者：韩小娇

通信作者：Zhuo, RY[1];Zhuo, RY[2];Li, JH[3]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China;[3]Shandong Agr Univ, Coll Forestry, Key Lab Agr Ecol & Environm, Tai An, Shandong, Peoples R China

年份：2016

卷号：14

期号：6

起止页码：1470-1483

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000378740200013)】；

基金：This work was supported by National High Technology Research and Development Program of China No. 2013AA102701-3, National Nonprofit Institute Research Grant of CAF (No. TGB2013008) and National Natural Science Foundation of China No. 31200465.

语种：英文

外文关键词：Sedum alfredii Hance; cadmium stress; phytoremediation; integration analysis; coexpression network

摘要：The hyperaccumulating ecotype of Sedum alfredii Hance is a cadmium (Cd)/zinc/lead co-hyperaccumulating species of Crassulaceae. It is a promising phytoremediation candidate accumulating substantial heavy metal ions without obvious signs of poisoning. However, few studies have focused on the regulatory roles of miRNAs and their targets in the hyperaccumulating ecotype of S. alfredii. Here, we combined analyses of the transcriptomics, sRNAs and the degradome to generate a comprehensive resource focused on identifying key regulatory miRNA-target circuits under Cd stress. A total of 87 721 unigenes and 356 miRNAs were identified by deep sequencing, and 79 miRNAs were differentially expressed under Cd stress. Furthermore, 754 target genes of 194 miRNAs were validated by degradome sequencing. A gene ontology (GO) enrichment analysis of differential miRNA targets revealed that auxin, redox-related secondary metabolism and metal transport pathways responded to Cd stress. An integrated analysis uncovered 39 pairs of miRNA targets that displayed negatively correlated expression profiles. Ten miRNA-target pairs also exhibited negative correlations according to a real-time quantitative PCR analysis. Moreover, a coexpression regulatory network was constructed based on profiles of differentially expressed genes. Two hub genes, ARF4 (auxin response factor 4) and AAP3 (amino acid permease 3), which might play central roles in the regulation of Cd-responsive genes, were uncovered. These results suggest that comprehensive analyses of the transcriptomics, sRNAs and the degradome provided a useful platform for investigating Cd hyperaccumulation in S. alfredii, and may provide new insights into the genetic engineering of phytoremediation.