文献类型：期刊文献

英文题名：Successful Establishment of Somatic Embryogenesis and Shoot Organogenesis Systems in Catalpa bungei C.A.Mey

作者：Sun, Jingshuang[1,2] Li, Jiewen[1,2] Zhao, Mengnan[3] Zheng, Guangshun[1,2] Zhang, Jing[3] Di, Bao[3] Ma, Wenjun[4] Wang, Junhui[4] Hu, Ruiyang[1,2]

第一作者：孙敬爽

通信作者：Hu, RY[1];Hu, RY[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Natl Forest Germplasm Resource Bank Representat Pl, Beijing 102300, Peoples R China;[3]Hebei Agr Univ, Hort Coll, Baoding 071001, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing 100091, Peoples R China

年份：2025

卷号：14

期号：17

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-105015994550);WOS:【SCI-EXPANDED(收录号:WOS:001569732800001)】；

基金：The study was supported by the Science Foundation of the Chinese Academy of Forestry (CAFYBB2020ZB004-2; CAFYBB2019MA001).

语种：英文

外文关键词：Catalpa bungei; regeneration; somatic embryogenesis; shoot organogenesis; histology; embryogenic callus

摘要：Catalpa bungei C.A.Mey is an economically significant deciduous tree valued for timber production and landscaping applications. An efficient regeneration system is crucial for clonal propagation and serves as a foundation for future molecular breeding in C. bungei. This study established two in vitro regeneration pathways-indirect somatic embryogenesis and shoot organogenesis utilizing mature zygotic embryos as explants. Primary callus was induced from cotyledon, hypocotyl, and plumule explants. A high frequency (45.73%) of yellow-green compact callus was achieved on De-Klerk and Walton (DKW) medium supplemented with 2.0 mg/L 6-BA, 1.0 mg/L zeatin (ZT), and 0.1 mg/L NAA. Subsequent transfer to 1.5x Murashige and Skoog (MS) medium containing 1.5 mg/L 6-BA, 0.2 mg/L ZT, and 0.1 mg/L NAA yielded the highest embryogenic callus induction rate (16.67%). Embryogenic callus demonstrated bipotent potential, generating both adventitious shoots and somatic embryos under specific hormonal conditions. Histological analyses confirmed the typical developmental stages of somatic embryos, from globular to cotyledonary forms, validating the embryogenic origin of regenerated structures. Furthermore, hormone or osmotic additives such as abscisic acid (ABA), Phytagel, and polyethylene glycol 4000 (PEG4000) significantly enhanced somatic embryo induction, with Phytagel at 5.0 g/L achieving the highest rate (76.31%). For shoot organogenesis, the optimal hormonal combination of the 0.6 mg/L 6-BA, 0.4 mg/L KT, and 0.15 mg/L NAA achieved the highest bud induction rate (88.89%) and produced an average of 4.07 adventitious buds per explant. This study presents an efficient regeneration system for C. bungei, providing a practical platform for large-scale propagation and basis for biotechnological applications in woody plants.