文献类型：期刊文献

英文题名：Insights into asynchronous changes of cell wall polymers accumulated in different cell types during conifer xylem differentiation

作者：Wang, Jie[1,2] Jia, Hao[3] Daniel, Geoffrey[4] Gao, Jie[4] Jiang, Xiaomei[1,2] Ma, Lingyu[1,2] Yue, Shuhua[3] Guo, Juan[1,2] Yin, Yafang[1,2]

第一作者：Wang, Jie

通信作者：Guo, J[1];Yin, YF[1];Yue, SH[2]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Natl Forestry & Grassland Adm, Wood Specimen Resource Ctr WOODPEDIA, Beijing 100091, Peoples R China;[3]Beihang Univ, Inst Med Photon, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Key Lab Biomech & Mechanob, Beijing 100191, Peoples R China;[4]Swedish Univ Agr Sci, Dept Forest Biomat & Technol Wood Sci, S-75007 Uppsala, Sweden

年份：2023

卷号：316

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20232314185924);Scopus(收录号：2-s2.0-85160687406);WOS:【SCI-EXPANDED(收录号:WOS:001014801400001)】；

基金：~This work was supported by the National Natural Science Foundation of China (grant no. 32071691) , and the National Forestry and Grassland Administration (grant no. 2020132601) . JW was supported by the China Scholarship Council (CSC grant no. 202003270032) . We would like to express our gratitude to Hao Jiang and Peng Miao in HORIBA Beijing for help with Confocal Raman tests, Chang Liu and Han Zhang of Beihang University for their help on SRS tests and Li Wang in Center of Institute of Biophysics, Chinese Academy of Science for her help of preparing TEM samples.

语种：英文

外文关键词：Xylem differentiation; Cambium; Tracheids; Rays; Cell wall polymers

摘要：An improved understanding of the events involved in cell wall polymers deposition during xylem development could provide new scientific ways for molecular regulation and biomass utilization. Axial and radial cells are spatially heterogeneous and have highly cross-correlated developmental behavior, whereas the deposition of corresponding cell wall polymers during xylem differentiation is less studied. To clarify our hypothesis that cell wall polymers of two cell types accumulated asynchronously, we performed hierarchical visualization, including label-free in situ spectral imaging of different polymer compositions during the development of Pinus bungeana. In axial tracheids, the deposition of cellulose and glucomannan was observed on earlier stages of secondary wall thickening than that of xylan and lignin, while xylan distribution was strongly related to spatial distribution of lignin during differentiation. The content of lignin and polysaccharides increased by over 130 % and 60 % respectively when the S3 layer was formed, compared to the S2 stage. In ray cells, the deposition of crystalline cellulose, xylan, and lignin was generally lagged compared to that in corresponding axial tracheids, although the process followed a similar order. The concentration of lignin and polysaccharides in ray cells was only approximately 50 % of that in the axial tracheids during secondary wall thickening.