文献类型：期刊文献

英文题名：Isolation and characterization of twenty-seven polymorphic microsatellite loci in endangered Chinese paperbark maple, Acer griseum (Dicotyledoneae: Aceraceae)

作者：Sun, Sheng[1] Yu, Xue-dan[1] Zheng, Yong-qi[1] Chen, Peng[1] Zhang, Chuan-hong[1]

第一作者：Sun, Sheng

通信作者：Zhang, CH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry, Beijing 100091, Peoples R China

年份：2014

卷号：56

起止页码：99-103

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;Scopus(收录号：2-s2.0-84901984167);WOS:【SCI-EXPANDED(收录号:WOS:000343385000016)】；

基金：This study was supported by special fund from State Forestry Administration in the Public Interest (201204307) and special fund from Central Public Welfare Scientific Research Institutes (RIF2010-01). We thank Bei-bei Cheng of Chinese Academy of Forestry for her help in sample collection and thank Huo-ran Wang of Chinese Academy of Forestry for his help in editing the abstract.

语种：英文

外文关键词：Maple; Acer griseum; Microsatellites; Polymorphism; Genetic diversity

摘要：Acer griseum is an endangered species in the world, while little is known about its genetic diversity. 27 polymorphic microsatellite loci from A. griseum were isolated and characterized by using a biotinylated (AG)(15)-enriched genomic library method. The polymorphisms of these markers were determined on 88 individuals. The number of alleles per locus ranged from 2 to 14. The observed heterozygosity, expected heterozygosity and the polymorphism information content ranged from 0384 to 0.897, 0.000 to 0.934, 0.436 to 0.877, respectively. It was found that 15 loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant genotypic linkage disequilibrium between pairs of loci. These microsatellites will be applied to further assessment of population genetic diversity and genetic resources conservation of A. griseum and other endangered maple species. (C) 2014 Elsevier Ltd. All rights reserved.