文献类型：期刊文献

中文题名：长嘴黄藤离体快繁研究

英文题名：Tissue Culture of Daemonorops jenkinsiana

作者：曾炳山[1] 刘英[1] 许煌灿[1] 尹光天[1]

第一作者：曾炳山

机构：[1]中国林科院热带林业研究所

年份：2002

卷号：22

期号：2

起止页码：169-171

中文期刊名：福建林学院学报

外文期刊名：Journal of Fujian College of Forestry

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD_E2011_2012】；

基金：国家自然科学基金资助项目"棕榈藤萌蘖机理研究" (3 9470 95 7)

语种：中文

中文关键词：离体快繁;长嘴黄藤;丛芽诱导;继代增殖;生根诱导;田间移植

外文关键词：Daemonorops jenkinsiana;tissue culture;proliferation;rooting;outplanting

分类号：S722.37

摘要：对长嘴黄藤组织培养的外植体选择、丛芽诱导、继代增殖、壮苗培养、生根诱导和田间移植进行研究 ,结果表明近成熟种胚萌发率高、褐变小、丛芽率高 ,为适宜的快繁外植体 .诱导丛芽的最佳激素组合与浓度为 BA2 .0～ 2 .2 mg/ L+ IBA0 .4mg/ L+ NAA0 .4mg/ L,每个母芽的新增殖芽数可达 1 5 .4.生根培养基可为 1 / 2 MS大量元素 + MS微量元素和有机质 + NAA0 .5mg/ L,丛芽苗生根率达 90 .9% .长嘴黄藤组培苗的木质化程度高 ,在高温低湿的秋季 ,移植成活率仍然可以达到 87% .
This paper deals with the selection of explant,induction of proliferation,subculture,rooting and outplanting of Daemonorops jenkinsiana,the results show that immature embryos are easy to proliferate and have little browning A hormone combination of BA2 2 mg/L, IBA0 4 mg/L and NAA0 4 mg/L is used to induce proliferation,in which proliferation coefficient can reach as high as 15 4 times per fifty days While rooting medium is half strength MS of huge elements and one MS of trace elements with organic matters and 0 5 mg/L of NAA,the rooting rate of one rooting culture is 90 9% Even in Autumn,the survival rate of outplanting can be higher than 87% The techniques can be used for mass propagation of D jenkinsiana seedlings