文献类型：期刊文献

中文题名：粗叶榕转录组SSR位点分析及分子标记开发

英文题名：Comprehensive Analysis of SSR Loci Within the Ficus hirta Vahl.Transcriptome and the Developmental Application of Molecular Markers

作者：燕青[1] 秦雅楠[1,3] 张华林[1] 李文秀[1] 叶亚娜[1] 黄安瀛[2] 罗萍[1]

第一作者：燕青

机构：[1]中国热带农业科学院湛江实验站/湛江市橡胶林下经济工程技术研究中心,广东湛江524013;[2]中国林业科学研究院速生树木研究所,广东湛江524300;[3]云南农业大学热带作物学院,云南普洱650201

年份：2025

卷号：46

期号：5

起止页码：1084-1093

中文期刊名：热带作物学报

外文期刊名：Chinese Journal of Tropical Crops

收录：;北大核心:【北大核心2023】；

基金：国家天然橡胶产业技术体系湛江综合试验站项目(No.CARS-33-GD2);广西自筹经费林业科技项目(No.2023GXZCLK13)。

语种：中文

中文关键词：粗叶榕;五指毛桃;转录组;EST-SSR;DNA指纹图谱

外文关键词：Ficus hirta Vahl.;Fici Hirtae Radix;transcriptome;EST-SSR;DNA fingerprinting

分类号：S567.19

摘要：五指毛桃(Fici Hirtae Radix)是一种中药材,其来源于粗叶榕(Ficus hirta Vahl.)的干燥根部。开发利用药用植物粗叶榕SSR分子标记,为其种质遗传多样性分析、遗传图谱构建、辅助育种等提供理论依据及数据支撑。以粗叶榕全缘、三裂、五裂、七裂4种典型叶片为试材,经转录组测序后进行位点特征分析及分子标记开发。结果表明:基于34375条转录组unigene序列,筛选到16 851个SSR位点,分布于12 094条unigene,分布频率为35.18%,平均每2638 bp有1个SSR位点,分布密度为3.68kb。共观察到5种不同核苷酸基序类型,其中最常见的是重复6次的基序,占SSR总数的20.92%,且长度基本小于29bp。双碱基重复序列占主导地位,其次是三碱基和四碱基重复类型。其中,(AG/CT)n是最常见的基元,占所有SSR的26.05%;其次是(AT/AT)n基元,占18.69%。随机选取100条EST-SSR引物,以粗叶榕4种不同叶型的基因组DNA扩增目标产物,其中89条引物在粗叶榕4个种质间表现出多态性。选取9对条带清晰的引物,对不同地区的31个粗叶榕种质进行DNA扩增和指纹图谱构建,这些引物组合多态性条带比例均值达72.04%,并对31个种质进行了有效鉴别,成功建立了DNA指纹图谱。研究结果证明粗叶榕转录组SSR位点信息丰富,利用性高,可为后续研究提供数据支撑。
Fici Hirtae Radix,a Chinese herbal medicine,is prepared from the dry roots of Ficus hirta Vahl.This study aimed to provide theoretical and empirical support for the genetic diversity analysis,genetic map construction,and as-sisted breeding of F.hirta Vahl.by developing SSR molecular markers.Four typical leaves of F.hirta Vahl.were se-lected as the samples,and site feature analysis and molecular marker development were performed after transcriptome sequencing.A total of 16851 SSR loci were identified from 34375 transcriptome unigene sequences,distributed across 12094 unigene sequences,resulting in a distribution frequency of 35.18%.The average frequency was one SSR locus every 2638 bp,with a density of 3.68 kb.Five distinct nucleotide motifs were observed,with the motif repeating six times being the most prevalent,constituting 20.92%of all SSRs,and typically less than 29 bp in length.Di-nucleotide repeats were predominant,followed by tri-and tetra-nucleotide repeats.(AG/CT)n was the most abundant repeat motif,representing 26.05%of all SSRs,while(AT/AT)n was the second most common,accounting for 18.69%.100 EST-SSR primers were randomly selected to amplify target product using genomic DNA from four different leaf types of F.hirta Vahl.89 primers exhibited polymorphism across the germplasms.Nine primer pairs,distinguished by the high definition and clarity,were selected to perform DNA amplification and construct DNA fingerprints for 31 specimens sourced from different regions.The average percentage of polymorphic bands observed across these primers was 72.04%.The sub-stantial degree of polymorphism facilitated the unambiguous identification of each of the 31 germplasm samples,and robust DNA fingerprint profiles were successfully established for each specimen.This study demonstrated that SSR loci within the transcriptome of F.hirta Vahl.were rich in information and highly utilizable,offering valuable data support for future research endeavors.