文献类型：期刊文献

英文题名：Microbial dynamics in a sequencing batch reactor treating alkaline peroxide mechanical pulp and paper process wastewater

作者：Zhan, Peng[1] Chen, Jienan[1,3] He, Gang[1] Fang, Guigan[2] Shi, Yingqiao[2]

第一作者：Zhan, Peng

通信作者：Chen, JN[1]

机构：[1]Cent S Univ Forestry & Technol, Inst Biol & Environm Sci & Technol, Changsha 410004, Hunan, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China;[3]Transpoints Inc, Gainesville, FL 32614 USA

年份：2010

卷号：17

期号：9

起止页码：1599-1605

外文期刊名：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

收录：;EI(收录号：20153201148735);Scopus(收录号：2-s2.0-77958144231);WOS:【SCI-EXPANDED(收录号:WOS:000282212900013)】；

基金：This work was supported by the National Key Science and Technology R&D Program of the 11th Five-Year Plan of China (grant no. 2006BAD18B0503).

语种：英文

外文关键词：Microbial dynamics; RAPD; APMP pulp and paper; SBR; Wastewater

摘要：Background, aim, and scope For many years, highly concentrated wastewater generated from the pulp and paper industry has become the focus of much concern worldwide. The objectives of this study were to determine the treatment efficiency of the alkaline peroxide mechanical pulp (APMP) process wastewater using a sequencing batch reactor (SBR) and analyze the microbial dynamics of the wastewater treatment system using the random amplified polymorphic DNA (RAPD) method. Materials and methods An SBR was applied to the treatment of APMP pulp and paper process wastewater. The wastewater characteristics and many physicochemical operator indicators in the wastewater treatment process were analyzed and determined according to standard methods. Microbial 16 S rDNA in active sludge was extracted, amplified, and analyzed using the RAPD method for the microbial dynamics of the wastewater treatment system. Results and discussion Ten kinds of natural organic compounds of plants such as monoterpene were detected in the APMP pulp and paper process wastewater. With an influent chemical oxygen demand (COD) that varied in the range of 685.7 to 907.5 mg/L, the corresponding effluent COD was 176.5 to 266.1 mg/L and the removal efficiency was 70.3% to 79.8%. An optimal strain (S308: CAGGGGTGGA) was selected to study the population dynamics and diversity of the bacterial community. The RAPD-polymerase chain reaction (PCR) fingerprints showed very high polymorphism of the genetic bands (78-100%). Four groups of species were clustered using the unweighted pair group method with arithmetic (UPGMA) analysis, and the genetic distance was close between the species within each group. The Shannon-Weaver index was high and varied over time with the COD removal. Conclusions The RAPD-PCR technique can be used to study microbial dynamics, which was shown to vary over time with the removal efficiency of SBR treating APMP pulp and paper process wastewater.