文献类型：期刊文献

英文题名：AFLP fingerprinting of Populus deltoides and Populus × canadensis elite accessions

作者：Gao, Jianming[1,2] Zhang, Yong[2,4] Wang, Chunguo[2] Zhang, Shougong[3] Qi, Liwang[3] Song, Wenqin[2]

第一作者：Gao, Jianming

通信作者：Song, W.

机构：[1] Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China; [2] Laboratory of Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China; [3] Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; [4] School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu 610054, China

年份：2009

卷号：37

期号：3

起止页码：333-344

外文期刊名：New Forests

收录：EI(收录号：20091612042615);Scopus(收录号：2-s2.0-64449083879)

基金：Acknowledgments This study was supported by grants from National Key Basic Research Program (‘‘973’’) (N2009CB119105), National Key Basic Research Program (‘‘973’’) (G19990160) and National ‘‘948’’ Program (N0.98-4-04-02).

语种：英文

外文关键词：Statistical methods

摘要：Amplified fragment length polymorphism (AFLP) was used in genetic fingerprinting of 22 elite Chinese Populus deltoides and Populus × canadensis accessions. The results indicated that each of the nine AFLP primer pairs selected generated fingerprint profiles that were unique to each of the accessions. Therefore, each accession was definitively identified by any of the nine primer pairs. Furthermore, the inter-accession genetic relationships inferred based on 461 polymorphic fragments from the nine AFLP primer pairs were largely consistent with phylogenetic relationships based on morphologic traits. Bootstrap analysis showed that three AFLP primer pairs were required to obtain genetic similarity values with a maximum CV of 10% while 10 AFLP primer pairs could give a maximum CV of 5%. Thus, AFLP can readily be applied for a rapid and accurate evaluation of the degree of similarity between poplar cultivars. In this study, the number of AFLP fragments used was sufficient to establish a reliable estimate of genetic similarity among accessions, with a maximum CV of 5.12%. Therefore, the information on the genetic relationships among the poplar accessions generated in this study in connection with knowledge on agronomic traits may have an impact on poplar breeding and planting in China. ? 2008 Springer Science+Business Media B.V.