文献类型：期刊文献

英文题名：Determination of 5 natural selenium species in selenium-enriched bamboo shoots using LC-ICP-MS

作者：Ni, Zhanglin[1] Liu, Yihua[1] Qu, Minhua[1]

第一作者：倪张林

通信作者：Liu, YH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China

年份：2014

卷号：23

期号：4

起止页码：1049-1053

外文期刊名：FOOD SCIENCE AND BIOTECHNOLOGY

收录：;EI(收录号：20143318055620);Scopus(收录号：2-s2.0-84905448483);WOS:【SCI-EXPANDED(收录号:WOS:000340357200007)】；

基金：This work was supported by the Special Funds from the Central Scientific Research Institute of Public Welfare (RISF61252), Special Fund for Forestry Scientific Research in the Public Interest (201304705).

语种：英文

外文关键词：selenium species; selenium-enriched; bamboo shoot; LC-ICP-MS

摘要：A method for simultaneous determination of the selenium species methylselenocysteine (MeSeCys), selenocysteine (SeCys(2)), selenite (SeIV), selenomethionine (SeMet), and selenates (SeVI) in natural selenium-enriched bamboo shoots was developed. Samples were extracted using sonication with a solution of pepsin. Different selenium species were separated using an anion exchange column with isocratic elution and a citric acid mobile phase at pH 4.7. Separated selenium species were detected as Se-80 using inductively coupled plasma mass spectrometry (ICPMS) in dynamic reaction cell (DRC) gas mode. The method achieved acceptable quantitative recoveries of 73.9 to 113.4% with relative standard deviations of < 5%. The limits of detection were 1, 2, 2, 3, and 3 mu g/kg (fresh weight) for SeCys(2), Se, MeSeCys, SeMet, and Se, respectively. The method was simple, rapid, accurate, and useful for determination of selenium species in natural selenium enriched bamboo shoots.