文献类型：期刊文献

英文题名：PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis

作者：Meng, Huijing[1,2] Zhao, Jinna[2] Yang, Yanfei[2] Diao, Kehao[2] Zheng, Guangshun[1] Li, Tao[2] Dai, Xinren[3] Li, Jianbo[1,3]

第一作者：Meng, Huijing

通信作者：Li, JB[1];Li, JB[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, China Natl Permanent Sci Res Base Warm Temperate Z, Beijing 102300, Peoples R China;[2]Shanxi Agr Univ, Coll Forestry, Taigu 030801, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2023

卷号：24

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001005650200001)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2021QB006 and CAFYBB2022QC001-2).

语种：英文

外文关键词：PeGSTU58; Populus; Arabidopsis; salt stress; drought stress

摘要：Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. Populus euphratica is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous study, PeGSTU58 was identified as being associated with seed salinity tolerance. In the present study, PeGSTU58 was cloned from P. euphratica and functionally characterized. PeGSTU58 encodes a Tau class GST and is located in both the cytoplasm and nucleus. Transgenic Arabidopsis overexpressing PeGSTU58 displayed enhanced tolerance to salt and drought stress. Under salt and drought stress, the transgenic plants exhibited significantly higher activities of antioxidant enzymes, including SOD, POD, CAT, and GST, compared to the wild-type (WT) plants. Additionally, the expression levels of several stress-responsive genes, including DREB2A, COR47, RD22, CYP8D11, and SOD1 were upregulated in PeGSTU58 overexpression lines compared to those in WT Arabidopsis under salt and drought stress conditions. Furthermore, yeast one-hybrid assays and luciferase analysis showed that PebHLH35 can directly bind to the promoter region of PeGSTU58 and activate its expression. These results indicated that PeGSTU58 was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by PebHLH35.