文献类型：期刊文献

英文题名：Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries

作者：Wang, Hui[1,2] Wang, Yue[1] Hou, Xiaopeng[2] Xiong, Benhai[1]

第一作者：Wang, Hui

通信作者：Wang, H[1];Xiong, BH[1];Wang, H[2]

机构：[1]Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China;[2]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China

年份：2020

卷号：10

期号：3

外文期刊名：NANOMATERIALS

收录：;Scopus(收录号：2-s2.0-85081255072);WOS:【SCI-EXPANDED(收录号:WOS:000526090400079)】；

基金：This research was funded by the Key Realm R and D Program of Guangdong Province (no. 2019B020215004), Science and Technology Major Project of Guangxi Province (GUIKE AA17204087-13), and the Chinese National Natural Science Foundation (no. 31671578).

语种：英文

外文关键词：bioelectronic nose; FET; ssDNA; SWNT; gas sensor; Phytophthora cactorum; volatile organic compounds; strawberry

摘要：The metabolic activity in plants or fruits is associated with volatile organic compounds (VOCs), which can help identify the different diseases. P-ethylphenol has been demonstrated as one of the most important VOCs released by the Phytophthora cactorum (P. cactorum) infected strawberries. In this study, a bioelectronic nose based on a gas biosensor array and signal processing model was developed for the noninvasive diagnostics of the P. cactorum infected strawberries, which could overcome the limitations of the traditional spectral analysis methods. The gas biosensor array was fabricated using the single-wall carbon nanotubes (SWNTs) immobilized on the surface of field-effect transistor, and then non-covalently functionalized with different single-strand DNAs (ssDNA) through pi-pi interaction. The characteristics of ssDNA-SWNTs were investigated using scanning electron microscope, atomic force microscopy, Raman, UV spectroscopy, and electrical measurements, indicating that ssDNA-SWNTs revealed excellent stability and repeatability. By comparing the responses of different ssDNA-SWNTs, the sensitivity to P-ethylphenol was significantly higher for the s6DNA-SWNTs than other ssDNA-SWNTs, in which the limit of detection reached 0.13% saturated vapor of P-ethylphenol. However, s6DNA-SWNTs can still be interfered with by other VOCs emitted by the strawberries in the view of poor selectivity. The bioelectronic nose took advantage of the different sensitivities of different gas biosensors to different VOCs. To improve measure precision, all ssDNA-SWNTs as a gas biosensor array were applied to monitor the different VOCs released by the strawberries, and the detecting data were processed by neural network fitting (NNF) and Gaussian process regression (GPR) with high accuracy.