文献类型：期刊文献

中文题名：核桃基因组DNA甲基化修饰位点的MSAP分析

英文题名：Analysis of Genomic DNA Methylation Modification of Walnut by Methylation-sensitive Amplification Polymorphism

作者：周贝贝[1] 文明[2] 马庆国[1] 裴东[1]

第一作者：周贝贝

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室;[2]凉山州林业局

年份：2014

卷号：27

期号：6

起止页码：741-744

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金项目(31171933)

语种：中文

中文关键词：核桃;MSAP;DNA甲基化;表观遗传学

外文关键词：Juglans regia; methylation-sensitive amplification polymorphism; DNA methylation; epigenetic

分类号：S718.46

摘要：以‘中宁强’核桃品种成熟叶片为材料,采用优化的MSAP反应体系,在全基因组水平对核DNA进行甲基化修饰位点分析,结果表明:选用20对引物组合,共扩增出1 060条清晰、重复性好的谱带,平均每对引物扩增出53条谱带,其中,甲基化位点241个,甲基化修饰比例为22.73%。对部分核桃DNA甲基化修饰位点进行回收测序,得到10条存在DNA甲基化修饰的DNA序列,BLASTn分析表明,核桃基因组中多种类型的DNA序列存在甲基化修饰现象。
Taking the mature leaves of the walnut variety Zhongningqiang＇ to analyze the level and patterns of DNA methylation modification loci on the whole genome of walnut by modified MSAP （methylation-sensitive amplification polymorphism）.By using 20 pairs of MSAP selective primers,a total of 1 060 legible and repeatable amplified DNA bands was detected,and 53 bands were observed after amplification with each primer pair in average.Of which,241 bands at ‘CCGG/GGCC’ loci,detected among all the amplified loci,were DNA methylated loci,with 22.73％of methylated modification in genome of walnut.Ten methylated DNA sequences were isolated and sequenced by extraction of the methylated loci.The results of BLASTn comparison showed that the DNA methylated modification phenomenon existed in multiple types of DNA sequence.This research,on methylation loci of genomicwide DNA in walnut,could provide references for the subsequent epigenetics research of walnut,as well as MSAP analysis of other woody plants.