文献类型：期刊文献

英文题名：The Salix psammophila SpRLCK1 involved in drought and salt tolerance

作者：Jia, Huixia[1] Li, Jianbo[2] Zhang, Jin[3] Sun, Pei[1] Lu, Mengzhu[1] Hu, Jianjun[1]

第一作者：Jia, Huixia

通信作者：Hu, JJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[3]Oak Ridge Natl Lab, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA

年份：2019

卷号：144

起止页码：222-233

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-85072770705);WOS:【SCI-EXPANDED(收录号:WOS:000496039500023)】；

基金：This work was supported by National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2017ZY008) and (CAFYBB2018ZY001-9), and Natural Science Foundation of China (31800570).

语种：英文

外文关键词：Receptor-like protein kinase; Transgene; Drought; Salt; Antioxidant enzyme; Salix psammophila

摘要：Receptor-like cytoplasmic kinases (RLCKs) play critical roles in biotic and abiotic stress responses in plants. However, the functions of RLCKs from the desert shrub willow Salix psammophila have not been characterized. Here, we focused on the biological function of SpRLCK1, which was previously identified as a potential drought-related gene. Phylogenetic analysis and subcellular localization revealed that SpRLCK1 was a cytoplasmic-localized protein with a protein kinase domain and belonged to the RLCK VIIa subclass. Gene expression profile revealed that SpRLCK1 was predominantly expressed in the root, being consistent with the GUS staining of pSpRLCK1:GUS transgenic plants. Additionally, the expression of SpRLCK1 was significantly induced by drought and salt stresses. To verify the function of SpRLCK1, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The SpRLCK1-overexpressing plants exhibited higher tolerance to drought and salt stresses, as evidenced by the higher survival rate, relative water content and antioxidant enzyme activity than those of wild-type plants. The SpRLCK1-overexpressing plants enhanced drought and salt tolerance by improving ROS-scavenging activities. A co-expression network for SpRLCK1 was constructed, and the expression analysis indicated that SpRLCK1 regulated the expression of a series of stress-related genes. Taken together, our results demonstrate that SpRLCK1 confers plant drought and salt tolerance through enhancing the activity of antioxidant enzymes and cooperating with stress-related genes.