文献类型：期刊文献

中文题名：楸树种子发育过程中内源激素含量的动态变化

英文题名：Dynamic Changes of Endogenous Hormone Content in Catalpa bungei Seeds During Their Developmental Periods

作者：韩东花[1,2] 杨桂娟[1] 肖遥[1] 麻文俊[1] 赵鲲[4] 王军辉[1] 易飞[1,3]

第一作者：韩东花

机构：[1]林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]北京师范大学珠海校区后勤处,广东珠海519087;[3]三峡大学生物与制药学院,湖北宜昌443002;[4]洛阳农林科学院,河南洛阳471023

年份：2021

卷号：34

期号：1

起止页码：56-64

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：“十三五”国家重点研发计划课题“楸树良种选育与高效培育技术研究”(2017YFD0600604)。

语种：中文

中文关键词：楸树;种子发育;内源激素

外文关键词：Catalpa bungei;seed development;endogenous hormones

分类号：S718.43

摘要：[目的]分析楸树种子发育过程中内源激素含量的动态变化,为探明楸树自花不育机理和在体胚诱导过程中添加外源激素的评估提供参考。[方法]以2个楸树无性系(洛楸1号和洛楸3号)授粉后不同时间的种子为材料,监测楸树胚胎发育过程,并采用液相色谱串联质谱法(LC-MS/MS)测定种子发育过程中IAA、iPA、GAs、ABA 4种内源激素含量的变化。[结果]洛楸3号种子的发育速度比洛楸1号的快;2个无性系种子IAA含量呈先下降后上升的趋势,但洛楸3号IAA含量在发育早期(授粉后45 d)即达到顶峰,而洛楸1号则在授粉后90 d才到达最大值;2个无性系种子iPA含量在发育过程中无显著变化,GAs和ABA含量呈持续下降趋势;2个无性系种子iPA/ABA、GAs/ABA和(IAA+iPA+GAs)/ABA的比值变化趋势一致,洛楸1号IAA/ABA的比值呈上升趋势,而洛楸3号IAA/ABA的比值先下降后上升。[结论]楸树种子发育过程中内源激素的含量随着胚胎发育而发生动态变化。IAA含量和IAA/ABA比值的差异是造成2个无性系胚胎发育速度差异的主要原因;早期胚胎发育需要较高的促进生长类激素,随后抑制生长类激素比例显著上升,促进胚胎与种子的成熟。
[Objective]To analyze the dynamic changes of endogenous hormone content during seed development of Catalpa bungei in order to reveal the mechanism of self-fertilization,as well as to provide evidence for adjusting the addition of exogenous hormones during somatic embryogenesis.[Method]The morphological changes of seeds of of two C.bungei clones(C.bungei'Luoqiu 1',and C.bungei'Luoqiu 3')were observed during their development after pollination.Their concentrations of IAA,iPA,GAs and ABA were analyzed using liquid chromatography tandem mass spectrometry(LC-MS/MS).[Result]The seed of clone C.bungei'Luoqiu 3'developed faster than that of clone C.bungei'Luoqiu 1'.The content of IAA in the two clones decreased at first and then increased,and the IAA content of clone C.bungei'Luoqiu 3'reached its peak at the early stage of development(the 45th day after pollination),while that of clone C.bungei'Luoqiu 1'reached its maximum value at 90th day after pollination.There was no significant change in iPA content in the seeds of the two clones,and the contents of GAs and ABA decreased continuously.The ratio of iPA/ABA,GAs/ABA and(IAA+iPA+GAs)/ABA of two clones showed the same trend,and the ratio of IAA/ABA of clone C.bungei'Luoqiu 1'was on the rise,while this ratio of clone C.bungei'Luoqiu 3'decreased at first and then increased.[Conclusion]The content of endogenous hormones of C.bungei seeds shows a dynamic change with the embryo development.The differences in IAA content and IAA/ABA ratio are the main reason for the difference of embryo development rate between the two clones.Early embryo development requires a high proportion of growth promoting hormones,followed by a significant increase in the proportion of growth inhibiting hormones,promoting embryo and seed maturation.