文献类型：期刊文献

中文题名：木荷侧枝形成过程解析及相关基因挖掘

英文题名：Analysis of Lateral Branches Formation in Schima superba and Exploration of Related Genes

作者：林子航[1,2] 姚进[3] 滕国新[3] 盛建洪[3] 范金根[3] 杜克久[1] 周志春[2] 张蕊[2]

第一作者：林子航

机构：[1]河北农业大学林学院,河北保定071000;[2]中国林业科学研究院亚热带林业研究所,浙江省林木育种技术重点实验室,浙江杭州311400;[3]浙江省兰溪市苗圃,浙江兰溪321100

年份：2025

卷号：38

期号：5

起止页码：37-48

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：;北大核心:【北大核心2023】；

基金：浙江省农业(林木)新品种选育重大科技专项重点项目(2021C02070-9)省市级;国家重点研发计划《南方主要珍贵树种新品种选育》(2024YFD2200102)。

语种：中文

中文关键词：木荷;侧芽;侧枝;内源激素;转录组

外文关键词：Schima superba;lateral bud;lateral branch;endogenous hormone;transcriptome

分类号：S792.99

摘要：[目的]研究常绿阔叶造林树种木荷侧枝形成的发育机制,挖掘分枝相关基因。[方法]本研究开展了木荷侧芽生长的表型观测,测定了休眠芽(S1)、萌动芽(S2)和幼枝(S3)3种状态的内源激素含量,利用转录组分析了差异基因的表达模式。[结果]木荷侧芽萌动并抽出新枝主要集中在3月上旬,侧枝生长在3月19日—4月9日出现1个侧枝发育“速生期”,二级枝前段(0~10 cm)总抽枝数量是后段(10~20 cm)的10倍以上,且福建产地的平均抽枝数量显著高于江西和浙江。内源激素含量GA3和BL的含量在S2和S3显著高于S1,在S2和S3中差异不显著;JA和ZR/IAA在S3显著高于S1和S2,在S1和S2中差异不显著。转录组显示,共152个差异基因参与了激素信号转导通路,WGCNA分析鉴定出3个与JA和BL相关的候选基因MYC2(Ssu06G00023)、BEH2(Ssu01G00918)和XTH23(Ssu07G01586)。[结论]木荷侧枝生长呈“慢-快-慢”的生长规律,抽枝主要集中在枝条上部0~10 cm,不同产地的平均抽枝数量存在显著差异。GA3和BL可能在促进木荷侧芽萌发中发挥重要作用,而JA和ZR/IAA可能在促进木荷抽枝过程中发挥重要作用。转录组挖掘出了3个与JA和BR相关的候选基因,蛋白互作网络进一步验证了其在木荷侧芽萌发和抽枝中的促进作用。
[Objective]To investigate the developmental mechanism of lateral branch formation in Schima superba,a species of evergreen broad-leaved afforestation tree,and to identify branching-related genes.[Methods]This study conducted phenotypic observations on the lateral bud growth of Schima superba,measured endogenous hormone contents at three stages:dormant buds(S1),sprouting bud(S2),and young shoots(S3),and analyzed the expression patterns of differential genes using transcriptome sequen-cing.[Results]The lateral buds of Schima superba initiated and produced new branches primarily in early March.A period of"rapid growth"in lateral branch development was observed between March 19th and April 9th.The total number of shoots on the front segment(0~10 cm)of secondary branches was more than 10 times that of the rear segment(10~20 cm).The average number of branches from Fujian was sig-nificantly higher than that from Jiangxi and Zhejiang.The contents of GA3 and BL were significantly higher in S2 and S3 than in S1,with no significant difference between S2 and S3.The contents of JA and ZR/IAA were significantly higher in S3 than in S1 and S2,with no significant difference between S1 and S2.The transcriptome analysis revealed that a total of 152 differential genes were involved in hormone signal transduction pathways.WGCNA analysis identified three candidate genes related to JA and BL:MYC2(Ssu06G00023),BEH2(Ssu01G00918),and XTH23(Ssu07G01586).[Conclusion]Lateral branch growth of Schima superba follows a"slow-fast-slow"pattern,with branching concentrated within the upper 0~10 cm of the branches.There are significant differences in the average number of branches among different origins.GA3 and BL play important roles in lateral bud germination,while JA and ZR/IAA maybe crucial regulators during the branching process of Schima superba.Three candidate genes related to JA and BR were identi-fied through transcriptome analysis,and protein interaction networks further validated their promoting roles in lateral bud germination and branching of Schima superba.