文献类型：期刊文献

中文题名：海南岛热带木本豆科植物根瘤菌的溶磷作用

英文题名：Phosphate Solubilization by Rhizobia Isolated from Woody Legume Plants in Hainan Island

作者：焦如珍[1] 彭玉红[1]

第一作者：焦如珍

机构：[1]中国林业科学研究院林业研究所

年份：2010

卷号：46

期号：10

起止页码：1-5

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家科技基础条件平台项目"林业微生物菌种资源标准化整理;整合及共享试点"子项目(2005DKA21207);国家科技支撑课题"高效好氧发酵菌和耐储存;高效能的微生物功能菌技术的研究集成"(2006BAD07A13-3)

语种：中文

中文关键词：木本豆科植物;根瘤菌;溶磷圈;溶磷能力;有效磷含量

外文关键词：tree legume; rhizobia; phosphorus-solubilized halo; phosphate solubilization; content of available P

分类号：Q143.2

摘要：对23株从海南尖峰岭自然保护区分离出的木本豆科植物根瘤菌及1株热带根瘤菌标准菌株进行溶磷圈定性试验及溶磷定量试验。结果表明:在以无机磷Ca3(PO4)2为唯一磷源的培养基内,有22株产生明显的溶磷圈;以Ca3(PO4)2为唯一磷源的培养液中有效磷含量超过平均值219.13mg·L-1的菌株有9株:caf333,caf443,caf336,caf278,caf344,caf444,caf276,caf436和caf341,溶磷能力最弱和最强的菌株分别为caf438和caf341,其培养液中的有效磷含量与对照相比分别增加了0.80和628.57mg·L-1;同一宿主、同一属种的根瘤菌不同菌株之间溶磷能力相差巨大;在以卵磷脂作为唯一磷源的培养基内,24株试验菌株均未出现溶磷圈。
In this experiment,solubilization of inorganic phosphorus and organic phosphorus by standard strain CGMCC 1. 2540T of Rhizobium tropici Martinez-Romero et al. and 23 rhizobia strains,which were isolated from woody legume plants in the Jianfengling Nature Reserve,Hainan Province,was investigated. By a qualitative test of phosphorus-solubilized halo,and quantitative test of content of available P in the medium with inorganic phosphorus of Ca3（PO4） 2 as the sole source of phosphorus,twenty-two strains were demonstrated to produce the phosphorus-solubilized halo,and there were significant differences in the diameter ratio between the halo and colony. The strains that produced the available phosphorus in the medium to be more than 219. 13 mg·L^-1 included 9 strains of caf 333,caf 443,caf 336,caf 278,caf 344,caf 444,caf 276,caf 436 and caf 341. The strain of caf 438 had the weakest capacity of phosphorus-solubilized while caf 341 had the strongest capacity to increase the available phosphorus in the medium,respectively by 0. 80 mg·L^-1 and 628. 57 mg·L^-1. Different strains of same species which were isolated from the same host showed significantly different capacity of phosphorus-solubilized. In the medium which lecithin was used as the sole source of phosphorus,all 24 strains tested did not displayed any phosphorus-solubilized halo.