文献类型：期刊文献

中文题名：基于高通量测序技术分析霉变核桃仁真菌多样性

英文题名：Analysis of Fungal Diversity of Moldy Walnut Kernel Based on High-throughput Sequencing Technology

作者：方圆[1] 苏高雅[2] 王卫雄[3] 白永超[2] 王丰俊[1] 裴东[2]

第一作者：方圆

机构：[1]林木资源高效生产全国重点实验室林业食品加工与安全北京市重点实验室北京林业大学生物科学与技术学院,北京100083;[2]林木遗传育种国家重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京100091;[3]东北林业大学生命科学学院,哈尔滨150040

年份：2023

卷号：23

期号：8

起止页码：369-378

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD;;EI(收录号：20234014839890);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家重点研发计划项目(2022YFD1000104-03);新疆维吾尔自治区重大科技专项(2022A02009-4)。

语种：中文

中文关键词：核桃;霉变;真菌;高通量测序;真菌群落多样性

外文关键词：walnut;mildew;fungi;high-throughput sequencing;fungal community diversity

分类号：TS2

摘要：目的:研究核桃仁在适宜霉变条件(温度25℃、相对湿度85%)下的真菌侵染过程,揭示其霉变过程中真菌群落的结构与真菌群落样性变化。方法:采用Illumina Miseq高通量测序方法分析核桃仁霉变过程中的真菌群落组成和多样性。结果:子囊菌门是核桃样品霉变的主要真菌门类,相对丰度在27.41%~99.98%范围,其次是担子菌门,相对丰度在0.01%~32.56%范围。在真菌属水平上,霉变过程中的主要优势种群为镰孢菌属、链格孢属、赤霉属与曲霉属。1周内核桃仁的霉变过程可大致分为Ⅰ期(0~2 d)、Ⅱ期(3~4 d)、Ⅲ期(5~7 d)3个时期,Ⅱ期与Ⅰ期相比镰孢菌属出现明显的富集,Ⅲ期与Ⅱ期相比镰孢菌属、链格孢属与曲霉属出现明显的富集,Ⅲ期与Ⅰ期相比镰孢菌属、链格孢属与曲霉属出现明显的富集。结论:核桃仁霉变过程中真菌微生物群落的变化分为3个时期,真菌多样性呈下降趋势,镰孢菌属、链格孢属与曲霉菌属是其霉变的优势真菌种群。
Objective:To reveal the changes of fungal community structure and diversity in walnut the potential health threats during natural mildew through studying the fungal infection process of walnut during natural mildew.Methods:The fungal ITS r DNA of fresh walnut kernel samples with different mildew time was sequenced by Illumina Miseq high-throughput sequencing technique,and the changes of fungal community composition and diversity in the process of fresh walnut kernel mildew were analyzed.Results:Ascomycota is the main fungal species in the mildew process of walnut samples,with a relative abundance of 27.41%-99.98%,followed by Basidiomycota with a relative abundance of 0.01%-32.56%.At the fungal level,it was found that the main dominant populations in the mildew process were Fusarium,Alternaria,Gibberella and Aspergillus.The mildew process of walnut in a week can be divided into three periods:PeriodⅠ(0-2 days),Period Ⅱ(3-4 days)and Period Ⅲ(5-7 days).In stages of Period Ⅰ and Period Ⅱ,Fusarium was the dominant genus,and the main fungus that change significantly among other periods were Fusarium,Aspergillus,Alternaria,Gibberella and Penicillium.Conclusion:The changes of fungal microbial community during mildew of walnut could be divided into three periods,during which the fungal diversity showed a downward trend,and there were a variety of harmful pathogens,such as Fusarium and Aspergillus,etc.,which might cause potential safety risks if not handled properly.