文献类型：期刊文献

英文题名：A Salt-Signaling Network Involving Ethylene, Extracellular ATP, Hydrogen Peroxide, and Calcium Mediates K+/Na+ Homeostasis in Arabidopsis

作者：Lang, Tao[1,2,3] Deng, Chen[4] Yao, Jun[4] Zhang, Huilong[4] Wang, Yin[1,2] Deng, Shurong[5]

第一作者：Lang, Tao

通信作者：Wang, Y[1];Wang, Y[2];Deng, SR[3]

机构：[1]Peking Univ, Inst Ecol, Coll Urban & Environm Sci, Minist Educ, Beijing 100871, Peoples R China;[2]Peking Univ, Minist Educ, Key Lab Earth Surface Proc, Beijing 100871, Peoples R China;[3]Chinese Res Inst Environm Sci, State Environm Protect Key Lab Reg Ecoproc & Func, Beijing 100012, Peoples R China;[4]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：21

期号：22

起止页码：1-20

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000594223400001)】；

基金：This work was jointly supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2019SY013, approval on 25 February 2019), the National Natural Science Foundation of China (Grant No. 31901287), and the Youth Fund of the Ministry of Education Laboratory for Earth Surface Processes, Peking University.

语种：英文

外文关键词：ethylene; eATP; H2O2; [Ca2+](cyt); NaCl; K+; Na+ homeostasis; Arabidopsis

摘要：This work aimed at investigating the interactive effects of salt-signaling molecules, i.e., ethylene, extracellular ATP (eATP), H2O2, and cytosolic Ca2+ ([Ca2+](cyt)), on the regulation of K+/Na+ homeostasis in Arabidopsis thaliana. The presence of eATP shortened Col-0 hypocotyl length under no-salt conditions. Moreover, eATP decreased relative electrolyte leakage and lengthened root length significantly in salt-treated Col-0 plants but had no obvious effects on the ethylene-insensitive mutants etr1-1 and ein3-1eil1-1. Steady-state ionic flux kinetics showed that exogenous 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) and eATP-Na-2 (an eATP donor) significantly increased Na+ extrusion and suppressed K+ loss during short-term NaCl treatment. Moreover, ACC remarkably raised the fluorescence intensity of salt-elicited H2O2 and cytosolic Ca2+. Our qPCR data revealed that during 12 h of NaCl stress, application of ACC increased the expression of AtSOS1 and AtAHA1, which encode the plasma membrane (PM) Na+/H+ antiporters (SOS1) and H+-ATPase (H+ pumps), respectively. In addition, eATP markedly increased the transcription of AtEIN3, AtEIL1, and AtETR1, and ACC treatment of Col-0 roots under NaCl stress conditions caused upregulation of AtRbohF and AtSOS2/3, which directly contribute to the H2O2 and Ca2+ signaling pathways, respectively. Briefly, ethylene was triggered by eATP, a novel upstream signaling component, which then activated and strengthened the H2O2 and Ca2+ signaling pathways to maintain K+/Na+ homeostasis under salinity.