文献类型：期刊文献

中文题名：农杆菌介导转化巨桉Eg5影响因素的研究

英文题名：Study on the factors influencing Agrobacterium tumefaciens mediated transformation of Eucalyptus grandis clone Eg5

作者：郭利军[1] 曾炳山[1] 刘英[1] 李湘阳[1] 裘珍飞[1]

第一作者：郭利军

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：32

期号：6

起止页码：61-66

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：863高新技术项目"白桦;桉树等分子育种与品种创制"项目编号(2011AA100202)

语种：中文

中文关键词：巨桉;农杆菌;Eg5;遗传转化

外文关键词：Eucalyptus grandis; Agrobacterium tumefaciens; Eg5; genetic transformation

分类号：S792-39;Q943.2

摘要：以巨桉无性系Eg5叶片为外植体材料,以GUS瞬时表达率和瞬时表达率指数作为评价指标,主要探讨了预培养时间、菌液浓度、侵染时间、共培养基pH、共培养时间和乙酰丁香酮（Acetosyringone,AS）对遗传转化的影响,结果表明预培养3 d、菌液浓度OD600 nm为0.5、接种30~60 min、共培养基pH值为5.8~6.0、共培养基中添加150 mg.L-1AS、菌液中不添加AS、共培养1 d遗传转化效果最佳,初步建立了巨桉Eg5的遗传转化体系。
By taking Eucalyptus grandis clone Eg5 leaves as explants, the GUS gene transient expression efficiency and transient expression efficiency index as evaluation indicators, effects of the pre-culture time, bacterial concentration, infection time, co-culture pH, co-culture time, Acetosyringone （AS） concentration in the bacterium liquid and Acetosyringone concentration in the medium on genetic transformation were investigated. The results show that pre-ctdture 3 days, 0.5 of OD600nm, 30-60 rain of infection time, 5.8,-6.0 of co-culture pH, 150 mg·L-t AS of the medium, and no AS in the bacterium liquid, lday of co-culture time, the best transformation effects were achieved. Thus, an effective system for Agrobacterium-mediated transformation of Eucalyptus grandis clone Eg5 was established.