文献类型：期刊文献

英文题名：Hormonal and morphological changes during seed development of Cypripedium japonicum

作者：Yan, Xiaona[1] Tian, Min[1] Liu, Fen[1] Wang, Caixia[1] Zhang, Ying[1]

通信作者：Tian, M[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, 73 Daqiao Rd, Fuyang 311400, Zhejiang, Peoples R China

年份：2017

卷号：254

期号：6

起止页码：2315-2322

外文期刊名：PROTOPLASMA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000413621600023)】；

基金：We thank Xiaoling Niu of Tianmu Mountain National Nature Reserve and Prof. Nianhang Rong of Zhejiang University for their help with these experiments. This work was supported by the National Science Foundation of China (grant number: 31370553) and the Key Science and Technology Specific Projects of Zhejiang Province (No. 2016C02056-13-2).

语种：英文

外文关键词：Cypripedium japonicum Thunb.; Morphological characteristics; Seed development; Endogenous hormones

摘要：With the goal of evaluating the factors that may be influencing the reproductive success of an endangered, terrestrial orchid Cypripedium japonicum Thunb., the anatomical and morphological characteristics of seed development were assessed, as were the levels of endogenous hormones to investigate the possible relationship between hormones and seed development. The results showed that morphological characteristics of seeds were changing from milky white and filiform to brown and fusiform. The development of ovule that initiated on the fertile valves only began after pollination, and a mature embryo sac was produced at 40 days after pollination (DAP). Fertilization occurred 50 to 60 DAP, and the newly formed zygote divided into two unequal cells that became the embryo proper and the suspensor. Seeds reached maturity at about 115 DAP, at which point they consisted only of a globular embryo and two layers of seed coat, without endosperm. The suspensor was lost, and only lipid particles and a large number of starch grains distributed in the cytoplasm. The peak of gibberellic acid (GA(3)), zeatin riboside (ZR), and indole acetic acid (IAA) occurred in a time sequence, as 20, 30, and 40 DAP. There was a highly significant positive correlation among them (P < 0.01). Abscisic acid (ABA) content increased at 60 DAP and reached the maximum at 115 DAP. ABA contents were significantly negatively correlated with the three hormones above (P < 0.05). It indicated that the lack of endosperm and suspensor tissues, delayed differentiation of embryos, and high levels of ABA may lead to the difficulty of germination.