文献类型：期刊文献

英文题名：INDUCTION OF ADVENTITIOUS SHOOTS FROM ROOT EXPLANTS IN BETULA ALNOIDES BUCH.-HAM. EX D. DON

作者：Wang, Huan[1] Guo, Junjie[1] Wang, Chunsheng[1] Yin, Haifeng[1] Dao, Baohui[2] Zeng, Jie[1]

第一作者：王欢

通信作者：Zeng, J[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, 682 Guangshanyi Rd,Longdong str, Guangzhou 510520, Peoples R China;[2]Res Inst Dehong Forestry, 202 Natl Rd 320, Ruili 678600, Yunnan, Peoples R China

年份：2025

卷号：25

期号：1

起止页码：3-10

外文期刊名：PROPAGATION OF ORNAMENTAL PLANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001503634400001)】；

基金：Acknowledgements : This study was supported by the research project of Jie Zeng Expert Workstation (202305AF150095) in Yunnan Province.

语种：英文

外文关键词：adventitious roots; adventitious shoots; Betulaceae; leaf with petiole; regeneration system

摘要：The optimal basal medium and plant growth regulators (PGRs) combinations for the induction of adventitious roots were identified using leaves with petioles of the clone of Betula alnoides (coded as TC2) as explants. Subsequently, segments of the adventitious roots were employed to research the effects of their position, dark culture duration, and PGRs combinations on the callus and adventitious shoots induction. The optimal medium for leaf rooting was WPM supplemented with 0.1 mg l(-1) NAA, 0.02 mg l(-1) BA, with rooting rate reaching 100% and above six roots after 30-day culture. The basal root section was identified as the most suitable segment, and dark culture for 10 days was determined to be the optimal time for callus induction. The callus induction rate reached 100% on medium WPB5 supplemented with 1.0-1.2 mg l(-1) TDZ and 0.2 mg l(-1) NAA. The adventitious shoots induction rate of root calluses was more than 60% on medium WPM supplemented with 1.2 mg l(-1) BA and 0.5 mg l(-1) GA(3). The total proliferation coefficient exceeded 0.8. The optimal scheme yielded approximately 0.9 adventitious shoots from each root callus of B. alnoides.