文献类型：期刊文献

英文题名：Transcriptomic analysis reveals importance of ROS and phytohormones in response to short-term salinity stress in Populus tomentosa

作者：Zheng, Lingyu[1] Meng, Yu[2] Ma, Jing[1] Zhao, Xiulian[1] Cheng, Telong[3] Ji, Jing[1] Chang, Ermei[1] Meng, Chen[4] Deng, Nan[1] Chen, Lanzhen[5,6] Shi, Shengqing[1] Jiang, Zeping[1]

第一作者：郑来友

通信作者：Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Agr Univ Hebei, Coll Landscape & Travel, Baoding, Peoples R China;[3]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing, Jiangsu, Peoples R China;[4]Tech Univ Munich, Chair Prote & Bioanalyt, Freisina, Germany;[5]Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100193, Peoples R China;[6]Minist Agr, Risk Assessment Lab Bee Prod Qual & Safety, Beijing, Peoples R China

年份：2015

卷号：6

期号：September

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000361305100001)】；

基金：This work was support by the Special Fund for Nonprofit Research Institute in Central Government (CAFYBB2012040), the National Natural Science Foundation of China (31100490; 31270707) and the Special Fund for Nonprofit Research Institute in Central Government (CAFYBB2014ZX001-3). We also thank Edanz copyediting for editing the manuscript.

语种：英文

外文关键词：Populus tomentosa; salt stress; transcriptomic analysis; differentially expressed genes; H2O2; hormone

摘要：Populus tomentosa (Chinese white poplar) is well adapted to various extreme environments, and is considered an important species to study the effects of salinity stress on poplar trees. To decipher the mechanism of poplar's rapid response to short-term salinity stress, we firstly detected the changes in H2O2 and hormone, and then profiled the gene expression pattern of 10-week-old seedling roots treated with 200 mM NaCl for 0, 6, 12, and 24 h (h) by RNA-seq on the Illumina-Solexa platform. Physiological determination showed that the significant increase in H2O2 began at 6 h. while that in hormone ABA was at 24 h, under salt stress. Compared with controls (0 h), 3991, 4603, and 4903 genes were up regulated, and 1408, 2206, and 3461 genes were down regulated (adjusted P <= 0.05 and vertical bar log2Ratio vertical bar >= 1) at 6, 12, and 24 h time points, respectively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation revealed that the differentially expressed genes (DEGs) were highly enriched in hormone- and reactive oxygen species-related biological processes, including "response to oxidative stress or abiotic stimulus," "peroxidase activity," "regulation of transcription," "hormone synthetic and metabolic process," "hormone signal transduction," "antioxidant activity," and "transcription factor activity." Moreover, K-means clustering demonstrated that DEGs (total RPKM value> 12 from four time points) could be categorized into four kinds of expression trends: quick up/down over 6 or 12 h, and slow up/down over 24 h. Of these, DEGs involved in H2O2- and hormone- producing and signal-related genes were further enriched in this analysis, which indicated that the two kinds of small molecules, hormones and H2O2, play pivotal roles in the short-term salt stress response in poplar. This study provides a basis for future studies of the molecular adaptation of poplar and other tree species to salinity stress.