文献类型：期刊文献

中文题名：Transfer DREB into Lolium perenne L. to improve its drought tolerance

英文题名：Transfer DREB into Lolium perenne L. to improve its drought tolerance

作者：Ma, Xinrong[2] Sun, Zhenyuan[3] Jiang, Changshun[1] Dong, Zhaoyong[1] Zhang, Yizheng[1]

第一作者：Ma, Xinrong

通信作者：Ma, X.

机构：[1] College of Life Sciences, Sichuan University, Chengdu 610064, China; [2] Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; [3] Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

年份：2006

卷号：12

期号：4

起止页码：427-433

中文期刊名：高技术通讯：英文版

外文期刊名：High Technology Letters

收录：EI(收录号：20070410388262);Scopus(收录号：2-s2.0-33846402109)

基金：Supported by the National Natural Science Foundation of China（30170589）and the National Special Project for Research and Industrialization of Transgenic Plants （J-2002-B-006）. Acknowledgements： We are grateful to Dr. Zhang Xiao-dong of Beijing Academy of Agricultural and Forestry Sciences for donating gene DRB1B and Agrobacterium tumefaciens EHA105. We thank Ms. Yang Hong of Chengdu Institute of Biology, Chinese Academy of Sciences, for conducting much work of tissue culture. We also thank Center for Application of Molecular Biology to International A griculture （CAMBIA）, Australia for permission of use pCAMBIA2301.

语种：英文

中文关键词：黑麦草;农杆菌;DREB基因;转录因子;双质粒;转基因技术;耐旱性;草坪草

外文关键词：Bacteria - Drought - Gene transfer - Genes - Identification (control systems) - Tissue culture - Vectors

分类号：S688.4;S543.6

摘要：A method of Agrobacterium tumefaciens mediated transformation for perennial ryegrass was developed using the calli of ryegrass derived from mature embryos. The calli were inoculated with a disarmed A. tumefaciens strain EHA105 harboring binary vector p2328. Vector p2328 contained transcription factor DREB1B and neomycin phosphotransferase (npt II) genes which were driven by promoters of rd29B and CaMV35S, respectively. The inoculated calli were selected on paromomycin-or kanamycin-containing media till the established plants being transferred to soil. Six transgenic plants with DREB1B had been obtained from perennial ryegrass strain Tove. PCR and Southern-blotting showed that npt II and DREB1B genes were integrated in perennial ryegrass genome. Stress treatment confirmed that transgenic plants with higher drought tolerance were obtained.