文献类型：期刊文献

英文题名：Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood

作者：Abbas, Manzar[1,2] Peszlen, Ilona[3] Shi, Rui[4] Kim, Hoon[5,6] Katahira, Rui[7] Kafle, Kabindra[8,9] Xiang, Zhouyang[10] Huang, Xiong[2] Min, Douyong[11] Mohamadamin, Makarem[8,9] Yang, Chenmin[12] Dai, Xinren[2] Yan, Xiaojing[2] Park, Sunkyu[3] Li, Yun[1] Kim, Seong H.[8,9] Davis, Mark[7] Ralph, John[5,6] Sederoff, Ronald R.[12] Chiang, Vincent L.[3,12,13] Li, Quanzi[2,14]

第一作者：Abbas, Manzar

通信作者：Li, QZ[1];Li, QZ[2]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]North Carolina State Univ, Dept Forest Biomat, Raleigh, NC 27695 USA;[4]North Carolina State Univ, Dept Crop & Soil Sci, Raleigh, NC 27695 USA;[5]Univ Wisconsin, Dept Biochem, Wisconsin Energy Inst, 420 Henry Mall, Madison, WI 53705 USA;[6]Univ Wisconsin, Wisconsin Energy Inst, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53705 USA;[7]NREL, Natl Bioenergy Ctr, Golden, CO 80401 USA;[8]Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA;[9]Penn State Univ, Mat Res Inst, University Pk, PA 16802 USA;[10]South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Peoples R China;[11]Guangxi Univ, Light Ind & Food Engn Coll, Nanning 530004, Peoples R China;[12]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA;[13]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[14]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：40

期号：1

起止页码：73-89

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000522853000007)】；

基金：This work was supported by grants from Fundamental Research Funds of the Chinese Academy of Forestry (CAFYBB2017MA030). NMR data was taken on instruments that were partly funded by the Department of Energy Great Lakes Bioenergy Research Center (DOE BER Office of Science DE-SC0018409). SFG analysis was carried out with the support from The Center for Lignocellulose Structure and Formation, Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Basic Energy Sciences, under Award Number DE-SC0001090.

语种：英文

外文关键词：cellulose; Populus trichocarpa; RNAi; secondary cell wall; wood formation

摘要：Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/8 and PtrCesA8-A/8 in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.