文献类型：期刊文献

英文题名：Identification of AUXIN RESPONSE FACTOR gene family from Prunus sibirica and its expression analysis during mesocarp and kernel development

作者：Niu, Jun[1] Bi, Quanxin[2] Deng, Shuya[1] Chen, Huiping[1] Yu, Haiyan[2] Wang, Libing[2] Lin, Shanzhi[3]

第一作者：Niu, Jun

通信作者：Niu, J[1]

机构：[1]Hainan Univ, Inst Trop Agr & Forestry, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing 10083, Peoples R China

年份：2018

卷号：18

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000423718500001)】；

基金：This study was supported by the Central Public Interest Scientific Institution Basal Research Fund (CAFYBB2016QA001), National Natural Science Foundation of China (31700586), and Hainan university research funded projects (KYQD(ZR)1701). These funding agencies had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

语种：英文

外文关键词：Prunus Sibirica; Auxin response factor; Expression profile; Co-expression analysis

摘要：Background: Auxin response factors (ARFs) in auxin signaling pathway are an important component that can regulate the transcription of auxin-responsive genes involved in almost all aspects of plant growth and development. To our knowledge, the comprehensive and systematic characterization of ARF genes has never been reported in Prunus sibirica, a novel woody biodiesel feedstock in China. Results: In this study, we identified 14 PsARF genes with a perfect open reading frame (ORF) in P. sibirica by using its previous transcriptomic data. Conserved motif analysis showed that all identified PsARF proteins had typical DNA-binding and ARF domain, but 5 members (PsARF3, 8 10, 16 and 17) lacked the dimerization domain. Phylogenetic analysis of the ARF proteins generated from various plant species indicated that ARFs could be categorized into 4 major groups (Class I, II, III and IV), in which all identified ARFs from P. sibirica showed a closest relationship with those from P. mume. Comparison of the expression profiles of 14 PsARF genes in different developmental stages of Siberian apricot mesocarp (SAM) and kernel (SAK) reflected distinct temporal or spatial expression patterns for PsARF genes. Additionally, based on the expressed data from fruit and seed development of multiple plant species, we identified 1514 ARF-correlated genes using weighted gene co-expression network analysis (WGCNA). And the major portion of ARF-correlated gene was characterized to be involved in protein, nucleic acid and carbohydrate metabolic, transport and regulatory processes. Conclusions: In summary, we systematically and comprehensively analyzed the structure, expression pattern and coexpression network of ARF gene family in P. sibirica. All our findings provide theoretical foundation for the PsARF gene family and will pave the way for elucidating the precise role of PsARF genes in SAM and SAK development.