文献类型：期刊文献

英文题名：PagJAZ5 regulates cambium activity through coordinately modulating cytokinin concentration and signaling in poplar

作者：Hu, Meng-Xuan[1,2] Guo, Wei[3] Song, Xue-Qin[1] Liu, Ying-Li[4] Xue, Yuan[4] Cao, Yuan[4] Hu, Jian-Jun[1] Lu, Meng-Zhu[1,2] Zhao, Shu-Tang[1,2]

第一作者：Hu, Meng-Xuan

通信作者：Lu, MZ[1];Zhao, ST[1];Lu, MZ[2];Zhao, ST[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[3]Taishan Acad Forestry Sci, Tai An 271000, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2024

卷号：243

期号：4

起止页码：1455-1471

外文期刊名：NEW PHYTOLOGIST

收录：;Scopus(收录号：2-s2.0-85196056936);WOS:【SCI-EXPANDED(收录号:WOS:001247491300001)】；

基金：This work was supported by grants from the National Science Foundation of China (32271902) and the State Key Laboratory of Subtropical Silviculture(SKLSS-KF2022-06).

语种：英文

外文关键词：cytokinin; JAZ; MYC2; poplar; type-A RR; wood formation; WOX4

摘要：center dot Wood is resulted from the radial growth paced by the division and differentiation of vascular cambium cells in woody plants, and phytohormones play important roles in cambium activity. center dot Here, we identified that PagJAZ5, a key negative regulator of jasmonate (JA) signaling, plays important roles in enhancing cambium cell division and differentiation by mediating cytokinin signaling in poplar 84K (Populus alba x Populus glandulosa). center dot PagJAZ5 is preferentially expressed in developing phloem and cambium, weakly in developing xylem cells. Overexpression (OE) of PagJAZ5m (insensitive to JA) increased cambium activity and xylem differentiation, while jaz mutants showed opposite results. Transcriptome analyses revealed that cytokinin oxidase/dehydrogenase (CKXs) and type-A response regulators (RRs) were downregulated in PagJAZ5m OE plants. The bioactive cytokinins were significantly increased in PagJAZ5m overexpressing plants and decreased in jaz5 mutants, compared with that in 84K plants. The PagJAZ5 directly interact with PagMYC2a/b and PagWOX4b. Further, we found that the PagRR5 is regulated by PagMYC2a and PagWOX4b and involved in the regulation of xylem development. center dot Our results showed that PagJAZ5 can increase cambium activity and promote xylem differentiation through modulating cytokinin level and type-A RR during wood formation in poplar.