文献类型：期刊文献

中文题名：吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响

英文题名：Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings

作者：刘昊[1,2] 宋晓波[1,2] 周乃富[1,2] 马庆国[1,2] 裴东[1,2]

第一作者：刘昊

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室;[2]中国林业科学研究院林木遗传育种国家重点实验室

年份：2017

卷号：34

期号：6

起止页码：1038-1043

中文期刊名：浙江农林大学学报

外文期刊名：Journal of Zhejiang A & F University

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家林业公益性行业科研专项(201304712);国家自然科学基金资助项目(31672126)

语种：中文

中文关键词：植物学;核桃;嫩枝扦插;不定根;高效液相色谱串联质谱法;植物激素

外文关键词：botany;walnut;softwood cutting;adventitious root;HPLC-MS;phytohormones

分类号：S718.4

摘要：植物激素是调控不定根形成的重要因素。通过测定胡桃Juglans复幼嫩枝扦插过程内源激素的质量分数变化,分析植物生长调节剂诱导对内源激素的影响,为探讨植物激素对不定根发生提供参考。以胡桃品种‘中宁盛’‘Zhongningsheng’复幼插穗为试验材料,利用高效液相色谱串联质谱法,测定外源吲哚丁酸(IBA)诱导处理下插穗基部内源激素变化,并统计生根情况。结果表明:外源IBA诱导处理对插穗生根影响显著。经IBA诱导的嫩枝插穗生根率高达98.1%,对照处理没有生根,死亡率达75.5%;发根过程经历了形成层增厚、分生组织细胞团产生、根原基初始细胞发生、根原基形成和根的发育等阶段,外部形态表现为增粗、开裂;与对照组相比,扦插后内源激素变化明显,吲哚乙酸(IAA)先升高,并于第5天达到峰值,之后降低;赤霉素(GA3)变化趋势与IAA一致;脱落酸(ABA)则是先降低后上升;玉米素(ZT)前期没有明显变化,根原基诱导后明显升高。外源生长素的诱导提高了嫩枝插穗IAA的水平,改变了内源激素质量分数,促进了不定根的形成。
Phytohormones are an important factor in regulating adventitious root formation. To provide a reference for discussing the role of phytohormones on adventitious root formation in walnut, the effect of exogenous hormones on endogenous hormones was analyzed by measuring the level of endogenous hormones during the cutting process of a rejuvenated softwood, Juglans（walnut） ‘Zhongningsheng’. The rejuvenated softwood was first induced by exogenous indole-3-butyric acid（IBA） and then, using High Performance Liquid Chromatography-Mass Spectrometry（HPLC-MS）, the endogenous hormones： Indole-3-acetic acid（IAA）, Gibberellic acid（GA3）, Abscisic Acid（ABA）, and Zeatin（ZT）; basal segments of soft-cuttings were measured followed by measurement of the rooting situation, student’s test were used for the treatment of the data. Results showed strong differences between samples induced and not induced by IBA. The rooting rate of rejuvenated softwood induced by IBA was up to 98.1%; whereas, in the control the rooting rate was 0 and the mortality rate was75.5%. The rooting process experienced several stages, such as thickening of the cambium, meristem cell formation, root primordia initial cell formation, root primordia formation, and root development through observation of the anatomic structure. Additionally, the external morphology showed thickening and cracking. During softwood cutting, endogenous hormone levels in rejuvenated soft shoots induced by IBA changed noticeably. Also,IAA increased first and reached its peak at day 5, then decreased as did GA3. However, ABA decreased first and then increased. In the early stages, ZT changed little but did increase after root primordia induction. The level of IAA in softwood induced by IBA improved; however, the effects on ZT were small. So the changes of endogenous hormones induced by IBA promoted adventitious root formation.