文献类型：期刊文献

英文题名：Metabolic Pathways Involved in the Drought Stress Response of Nitraria tangutorum as Revealed by Transcriptome Analysis

作者：Liu, Chenggong[1,2] Duan, Na[3,4] Chen, Xiaona[3,4] Li, Huiqing[1,2] Zhao, Xiulian[1,2] Duo, Puzeng[3,4] Wang, Ji[5] Li, Qinghe[1,2]

第一作者：Liu, Chenggong

通信作者：Li, QH[1];Li, QH[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Bayan Nur 015200, Peoples R China;[4]Natl Forestry & Grassland Adm, Ulan Buh Desert Comprehens Control Natl Permanent, Bayan Nur 015200, Peoples R China;[5]Inner Mongolia Agr Univ, Coll Desert Control Sci & Engn, Hohhot 010010, Peoples R China

年份：2022

卷号：13

期号：4

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000787527700001)】；

基金：This research was supported by the National Natural Science Foundation of China (Grant No. 31470622), the National Key R&D Program of China during the 13th Five-year Plan Period (Grant No. 2019YFF030320102), and the National Forest Germplasm Resource Platform Construction and Operation Services (Grant No. 2005DKA21003).

语种：英文

外文关键词：transcriptome analysis; drought stress; Nitraria tangutorum; metabolic pathways

摘要：Drought resistance in plants is controlled by multiple genes. To identify the genes that mediate drought stress responses and to assess the associated metabolic pathways in the desert shrub Nitraria tangutorum, we conducted a transcriptome analysis of plants under control (maximum field capacity) and drought (20% of the maximum field capacity) conditions. We analyzed differentially expressed genes (DEGs) of N. tangutorum and their enrichment in the KEGG metabolic pathways database, and explored the molecular biological mechanisms underlying the answer to its drought tolerance. Between the control and drought groups, 119 classified metabolic pathways annotated 3047 DEGs in the KEGG database. For drought tolerance, nitrate reductase (NR) gene expression was downregulated, indicating that NR activity was decreased to improve drought tolerance. In ammonium assimilation, drought stress inhibited glutamine formation. Protochlorophyllide reductase (1.3.1.33) expression was upregulated to promote chlorophyll a synthesis, whereas divinyl reductase (1.3.1.75) expression was downregulated to inhibit chlorophyll-ester a synthesis. The expression of the chlorophyll synthase (2.5.1.62) gene was downregulated, which affected the synthesis of chlorophyll a and b. Overall, drought stress appeared to improve the ability to convert chlorophyll b into chlorophyll a. Our data serve as a theoretical foundation for further elucidating the growth regulatory mechanism of desert xerophytes, thereby facilitating the development and cultivation of new, drought-resistant genotypes for the purpose of improving desert ecosystems.