文献类型：期刊文献

英文题名：Transcriptome analysis of the genes and regulators involving in vitamin E biosynthesis in Elaeagnus mollis diels

作者：Du, Shuhui[1] Guo, Yuanting[1] Li, Qianqian[1] Hu, Xiaoyan[1] Tian, Yang[2] Cheng, Baochang[2] Wang, Shengji[1] Wang, Zhiling[1] Ren, Ruifen[1] Wang, Zhaoshan[2]

第一作者：Du, Shuhui

通信作者：Du, SH[1];Wang, ZS[2]

机构：[1]Shanxi Agr Univ, Coll Forestry, Jinzhong, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture State Forestry Adm, Beijing, Peoples R China

年份：2024

卷号：114

期号：6

外文期刊名：PLANT MOLECULAR BIOLOGY

收录：;Scopus(收录号：2-s2.0-85206700112);WOS:【SCI-EXPANDED(收录号:WOS:001335881800001)】；

基金：This work was supported by the Fundamental Research Program in Shanxi Province (20210302124175), Special sub-project of Basic Science and Technology Resources Survey of the Ministry of Science and Technology (2019FY100802_04) and Innovation Project of Shanxi Agricultural University (2016YJ12).

语种：英文

外文关键词：Elaeagnus mollis; Vitamin E; VTE gene; Transcription factor EmWRKY13

摘要：Elaeagnus mollis is an important newly developing woody oil plant species and the vitamin E (VitE) content in its kernel oil is relatively high. In the present study, the VitE component content and functional genes involving in VitE biosynthesis in E. mollis kernel at different developmental stage were investigated. The VitE content increased with kernel development, reaching up to similar to 7.96 mg/g oil in kernel mature stage. The content of tocopherol was much higher than that of tocotrienol and gamma-tocopherol became the dominant component. E. mollis kernel extracts had relatively strong antioxidant capacity. We identified 17 genes (16 VTEs and 1 homogentisic acid geranylgeranyl transferase (HGGT)) directly involving in VitE biosynthesis in RNA-Seq data. Phylogenetic and qRT-PCR results indicated that the annotation and reliability of the RNA-Seq were accurate. Transient overexpression of EmVTE3 and EmWRKY13 in tobacoo leaves increased and decreased the VitE content to 192.18 and 118.29 mu g/g, respectively. Weighted gene co-expression analysis elucidated that the blue module showed significant correlation with tocopherol content. Co-expression network analysis revealed that 2-methyl-6-phytobenzoquinone methyltransferase (MPBQ-MT/VTE3) played a vital role and EmWRKY13 may be a key negative regulator in E. mollis VitE biosynthesis. This study not only revealed the traditional VitE biosynthesis pathway in E. mollis, but also set a solid foundation for future genetic breeding of this species.