文献类型：期刊文献

英文题名：Assessment of genetic diversity of Camellia yuhsienensis based on leaf structure and inter simple sequence repeat (ISSR) markers

作者：Zou, Yuling[1] Ge, Xiaoning[1] Yan, Chao[1] Zhong, Qiuping[1] Chen, Danyang[1] Chen, Zexin[1] Yuan, Yaqi[1] Guo, Hongyan[1] Zhou, Youcheng[1] Wang, Jinfeng[1] Cao, Linqing[1]

第一作者：邹玉玲

通信作者：Zhong, QP[1];Cao, LQ[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China

年份：2024

外文期刊名：GENETIC RESOURCES AND CROP EVOLUTION

收录：;Scopus(收录号：2-s2.0-85188048199);WOS:【SCI-EXPANDED(收录号:WOS:001187435000003)】；

基金：This research was supported by Fundamental Research Funds for the Central Non-profit Research Institution (CAFYBB2021MA003, CAFYBB2023ZA001-2, CAFYBB2019MB003)

语种：英文

外文关键词：Camellia yuhsienensis Hu; Leaf morphology; ISSR marker; Cluster analysis; Genetic diversity

摘要：Genetic diversity provides valuable genetic resources and forms the basis of plant breeding. Plant leaves are closely connected to the external environment and play important roles in plant growth and development. Here, 13 cultivars of Camellia yuhsienensis were collected from Southern China to examine the magnitude of genetic diversity and differentiation, using cluster analysis based on 21 leaf traits and ten Inter simple sequence repeat (ISSR) markers. Morphological study indicated that all traits varied to different degrees, indicating a high degree of genetic diversity in C. yuhsienensis. Eight typical leaf characteristics were screened using principal component analysis, which showed that the selected traits were highly correlated and clustered. Average polymorphism percentage was obtained from the ISSR marker pattern using ten primers. A total of 201 loci were amplified, of which 175 were polymorphic with an average polymorphism percentage of 87.06%. Polymorphism information content (PIC), marker index (MI) and resolving power (RP) revealed that the selected primers were found to be informative in distinguishing C. yuhsienensis germplasm resources. Nei's diversity Index and the Shannon's diversity index values indicated a certain level of genetic diversity. Cluster analysis results based on leaf structure and molecular markers were generally consistent, indicating a high degree of effective loci for morphological markers of C. yuhsienensis. Our results revealed genetic differences and commonalities among the cultivars, providing valuable information to identify appropriate seedling cultivars and conserve germplasm resources for future utilization.