文献类型：期刊文献

英文题名：Effect of Overexpression ofJERFson Intracellular K+/Na(+)Balance in Transgenic Poplar (Populus albaxP. berolinensis) Under Salt Stress

作者：Ding, Changjun[1,2] Zhang, Weixi[1,2] Li, Dan[1,2] Dong, Yufeng[3] Liu, Junlong[4] Huang, Qinjun[1,2] Su, Xiaohua[1,2]

第一作者：丁昌俊

通信作者：Huang, QJ[1];Su, XH[1];Huang, QJ[2];Su, XH[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, Beijing, Peoples R China;[3]Shandong Acad Forestry, Shandong Prov Key Lab Forest Tree Genet Improveme, Jinan, Peoples R China;[4]Anhui Acad Forestry, Ind Timber & Bamboo, Hefei, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000566106900001)】；

基金：This research was funded by the National Major Project of GMO New Species Cultivation (Grant No. 2018ZX08020002) and the National High-Tech R&D Program of China (863 Program) (Grant No. 2011AA100201).

语种：英文

外文关键词：poplar; JERF36s; ion balance; NHX1; SOS1; salt resistance

摘要：Salt stress is one of the main factors that affect both growth and development of plants. Maintaining K+/Na(+)balance in the cytoplasm is important for metabolism as well as salt resistance in plants. In the present study, we monitored the growth (height and diameter) of transgenicPopulus albaxP. berolinensistrees (ABJ01) carryingJERF36sgene (a tomato jasmonic/ethylene responsive factors gene) over 4 years, which showed faster growth and significant salt tolerance compared with non-transgenic poplar trees (9#). The expression ofNHX1andSOS1genes that encode Na+/H(+)antiporters in the vacuole and plasma membranes was measured in leaves under NaCl stress. Non-invasive micro-test techniques (NMT) were used to analyse ion flux of Na+, K+, and H(+)in the root tip of seedlings under treatment with100 mM NaCl for 7, 15, and 30 days. Results showed that the expression ofNHX1andSOS1was much higher in ABJ01 compared with 9#, and the Na(+)efflux and H(+)influx fluxes of root were remarkable higher in ABJ01 than in 9#, but K(+)efflux exhibited lower level. All above suggest that salt stress inducesNHX1andSOS1to a greater expression level in ABJ01, resulting in the accumulation of Na+/H(+)antiporter to better maintain K+/Na(+)balance in the cytoplasm of this enhanced salt resistant variety. This may help us to better understand the mechanism of transgenic poplars with improving salt tolerance by overexpressingJERF36sand could provide a basis for future breeding programs aimed at improving salt resistance in transgenic poplar.