文献类型：期刊文献

中文题名：美洲黑杨雄性花芽cDNA克隆测序及表达序列标签(ESTs)特性分析

英文题名：Sequencing of cDNA Clones and Analysis of the Expressed Sequenced Tags(ESTs)Properties of Male Floral Buds of Populus deltoides

作者：周祥明[1] 张冰玉[2] 苏晓华[2] 王大海[2] 黄秦军[2] 张香华[2] 张志毅[1]

第一作者：周祥明

机构：[1]北京林业大学林木花卉遗传育种教育部重点开放实验室;[2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2006

卷号：42

期号：11

起止页码：37-41

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(30200225)

语种：中文

中文关键词：美洲黑杨;雄性花芽;cDNA文库;表达序列标签

外文关键词：Populus deltoides ; male floral bud; cDNA library; ESTs

分类号：S718.46;Q943.2

摘要：采用SMART技术构建美洲黑杨雄性花芽cDNA文库。随机挑选4200个克隆进行序列测定,获得原始序列3092个ESTs,去除插入片段短于150bp的序列和污染序列后,获得有效ESTs序列3087个,平均长度515bp。对聚类后的416个Clusters分别进行单独拼接,得到相应的Contigs和Singletons分别为451和1104个,并通过与NCBI等核酸数据库进行比对、查询和注释,获得已知功能和未知功能的基因1015个,无序列相似性的新基因540个。这些数据为进一步研究高等植物花发育提供了一个极有价值的资源。
A cDNA library of male floral buds of Populus deltoides through SMART（the Switch Mechanism At the 5＇ end of RNA Templates）technique was constructed. 4 200 clones randomly selected from the cDNA library were sequenced and 3 092 raw sequences were obtained. 3 087 valid ESTs were generated after the size of inserts shorter than 150 bp and the contaminants were removed. The average length of the cDNA was 515 bp. A total of 416 clusters were formed after assembly of the 3 087 ESTs, and 451 contigs and 1 104 singletons were obtained. 1 015 ESTs with known or unknown function were identified by BlastN searched against the NcBI non-redundant nucleotide databases, 540 ESTs had no significant similarity to any protein or DNA sequence in the databases. The present ESTs provide a valuable resource for further research on the flower development of higher plants.