文献类型：期刊文献

中文题名：杞柳4个品种Cd的亚细胞分布、化学形态及其对Cd转运的影响

英文题名：Subcellular distribution,chemical forms of cadmium and the effects on cadmium transportation in four cultivars of Salix integra

作者：舒婉钦[1,2] 陈光才[2] 王树凤[2] 曹基武[1]

第一作者：舒婉钦

机构：[1]中南林业科技大学林学院,长沙410004;[2]中国林业科学研究院亚热带林业研究所,杭州311400

年份：2022

卷号：58

期号：9

起止页码：1766-1778

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金(31770653)。

语种：中文

中文关键词：镉;杞柳;转运;亚细胞分布;化学形态

外文关键词：cadmium;Salix integra;transportation;subcellular distribution;chemical forms

分类号：Q945.78;X173

摘要：为明晰杞柳(Salix integra)不同品种对镉(Cd)的积累和分配特征及其Cd转运效率的影响因素,采用水培方法,研究了杞柳4个栽培品种(‘大红头’、‘大青皮’、‘黄皮柳’和‘庄坞柳’)在低浓度(10μmol·L^(-1))和高浓度(50μmol·L^(-1))Cd胁迫下,不同组织Cd的积累和转运特征、亚细胞分布及化学形态。结果发现,杞柳体内的Cd主要积累在根部,其次为叶片,新枝和插条Cd含量最低;不同组织Cd含量随着Cd处理浓度的增加而增加,但不同品种之间差异不显著。在亚细胞水平,根部和叶片的Cd大多贮存在细胞壁和可溶性组分,但在高浓度Cd胁迫下,‘大红头’、‘黄皮柳’和‘庄坞柳’叶片Cd向膜及细胞器组分的分配比例增加。进一步对组织内Cd的化学形态进行分析,发现叶片中61.32%~80.34%的Cd以氯化钠提取态和醋酸提取态存在,随着Cd浓度的增加,移动性和毒性较强的乙醇提取态和去离子水提取态Cd含量显著增加;根部平均57.39%的Cd以乙醇提取态存在,其次为去离子水提取态、氯化钠提取态和醋酸提取态,盐酸提取态含量最低,仅占0.52%~4.91%。4个品种根系Cd向叶片的转运系数均大于新枝和插条,通过对转运系数与Cd的亚细胞分布和化学形态相关性分析发现,Cd向叶片的转运系数与叶中氯化钠提取态的Cd含量以及叶片膜及细胞器组分中的Cd显著相关,表明果胶和蛋白质结合态Cd不仅缓解Cd对叶片的毒害,而且可能有助于Cd向叶片的转移和积累。
In order to explore the characteristic of cadmium(Cd)accumulation and transportation in Salix integra,a hydroponic experiment was conducted to study the accumulation and translocation characteristics,subcellular distribution and chemical forms of Cd in different tissue in four cultivars of S.integra(‘Dahongtou’,‘Daqingpi’,‘Huangpiliu’and‘Zhuangwuliu’)under lower(10μmol·L^(-1))and higher(50μmol·L^(-1))levels of Cd exposure.The results showed that the content of Cd in different cultivars was mainly accumulated in roots,followed by leaves,and the lowest in new shoots and cuttings.The Cd content in different tissues increased with the increased level of Cd treatment,and there were no significant differences among different cultivars.Subcellular fractionation of Cd-containing tissues indicated that both in roots and leaves,the majority of Cd was located in the cell wall and soluble fraction.However,under the stress of higher level of Cd,the distribution proportion of Cd to the membrane and organelle components in leaves of‘Dahongtou’,‘Huangpiliu’and‘Zhuangwuliu’increased.Further analysis of the chemical forms of Cd in tissues showed that 61.32%-80.34% Cd in leaves existed as NaCl-and HAc-extracted forms.With the increase of Cd level,the content of Cd in the extraction of ethanol and d-HO,which are highly mobile and toxic,increased significantly.57.39% of Cd in root was extracted by ethanol,followed by extracted with d-HO,NaCl and HAc,and the content of Cd in the extraction of HCl was the lowest,accounting for only 0.52%-4.91%.The translocation factors of Cd from root to leaf of four Salix cultivars was greater than those of new shoot and cutting.By analyzing the relationship between the translocation factors and the subcellular distribution and chemical forms of Cd,it was found that the translocation factors of Cd to leaf was significantly correlated with the content of Cd in the extraction of NaCl in leaf and the content of Cd in membrane and organelle in leaf.It indicated that the pectin and protein-bound Cd not only relieves the toxicity of Cd to the leaves,but may also help the transfer and accumulation of Cd to leaf.