文献类型：期刊文献

中文题名：不同木质纤维素基质对白腐真菌固态发酵产漆酶的影响

英文题名：Effects of different lignocellulosic substrates on laccase production by white-rot fungi in solid-state fermentation

作者：边禄森[1] 安琪[2] 刘慧慧[1] 苗朔[1] 杨依宁[2] 李施漫[2] 王兴红[1] 韩美玲[2]

第一作者：边禄森

机构：[1]中国林业科学研究院华北林业实验中心北京九龙山暖温带森林国家长期科研基地,北京102300;[2]廊坊师范学院生命科学学院,河北廊坊065000

年份：2025

卷号：44

期号：11

起止页码：223-235

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：;北大核心:【北大核心2023】；

基金：中国林科院中央级公益性科研院所基本科研业务费(CAFYBB2021MA007);河北省自然科学基金(C2024408007)。

语种：中文

中文关键词：愈创木酚;液态发酵;阔叶树;油松松针;漆酶活性

外文关键词：guaiacol;submerged fermentation;broad-leaved tree;needles of Pinus tabuliformis;laccase activity

分类号：TQ925

摘要：本文对新分离得到的24株白腐真菌进行愈创木酚培养基初筛,获得的菌株进行液体发酵产漆酶能力分析和不同木质纤维素基质上固态发酵产漆酶能力分析。结果表明,12株白腐真菌在液态发酵条件下所检测到的漆酶活性和其菌丝圈直径与变色圈直径的比值无对应关系(P>0.05);同样,不同真菌在不同木质纤维素基质上固体发酵时所检测到的漆酶活性与其菌丝圈直径与变色圈直径的比值无对应关系(P>0.05)。液态发酵过程中,一色齿毛菌Cerrena unicolor LKY 798、毛栓孔菌Trametes hirsuta LKY 831、硬毛粗盖孔菌Funalia trogii LKY 846和大孔新棱孔菌Neofavolus alveolaris LKY 850漆酶活性较高且高漆酶活性时间较长。在以栓皮栎树叶、北京杨树叶和油松松针为基质进行固态发酵时,N.alveolaris LKY 850和Trametes sp.LKY 797产漆酶能力最强。总体而言,栓皮栎树叶和北京杨树叶相较于油松松针更适宜于真菌固态发酵产漆酶。此外,Trametes sp.LKY 797、F.trogii LKY 846和N.alveolaris LKY 850在油松松针为底物发酵时,漆酶活性较好,最大漆酶活性均超过3700 U/mL。
Twenty-four newly isolated strains of white-rot fungi were cultured on guaiacol-supplemented medium for screening laccase-producible strains,the capacity of laccase production of the 12 strains obtained was analysed in submerged fermentation and solid-state fermentation on different lignocellulosic substrates.The results indicated that there was no significant correlation between the laccase activity detected under submerged fermentation conditions and the ratio of colony diameters to photochromic laps among the 12 test strains(P>0.05).Similarly,no significant association was observed between the laccase activity detected during solid-state fermentation on different lignocellulosic substrates and the ratio of colony diameters to photochromic laps for the various test strains(P>0.05).In submerged fermentation,Cerrena unicolor LKY 798,Trametes hirsuta LKY 831,Funalia trogii LKY 846,and Neofavolus alveolaris LKY 850 exhibited comparatively high laccase activities keeping for a fairly long duration.N.alveolaris LKY 850 and Trametes sp.LKY 797 demonstrated the strongest laccase production capacities under solid-state fermentation condition using leaves of Quercus variabilis,Populus beijingensis,and needles of Pinus tabuliformis as substrates.It seems that leaves of Quercus variabilis and Populus beijingensis are more suitable than needles of Pinus tabuliformis for laccase production under solid-state fermentation condition.Additionally,Trametes sp.LKY 797,F.trogii LKY 846,and N.alveolaris LKY 850 showed relatively good laccase activities in fermentation using needles of Pinus tabuliformis as the substrate,with maximum laccase activities exceeding 3700 U/mL.