文献类型：期刊文献

中文题名：欧美杨溃疡病的病原鉴定

英文题名：Pathogen identification of Populus × euramericana Canker Disease

作者：贺伟[1] 任飞娟[1] 郭利民[2] 李永[1,3] 常聚普[2]

第一作者：贺伟

机构：[1]北京林业大学森林培育与保护教育部重点实验室;[2]河南省濮阳市林业科学研究所;[3]中国林业科学研究院森林生态环境与保护研究所

年份：2009

卷号：45

期号：6

起止页码：104-108

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家十一五科技支撑计划课题(2006BAD32B02);河南省林木重大病虫害成灾机理及可持续控制技术研究(081100911400);长江学者与创新团队发展计划(PCSIRT0607);国家科技基础条件平台项目(2005DKA21207-7)

语种：中文

中文关键词：欧美杨溃疡病;病原菌;茄镰孢菌

外文关键词：Populus× euramericana canker disease; pathogen; Fusarium solani

分类号：S763.7

摘要：从河南濮阳、山东菏泽等地的欧美杨溃疡病发病林地采集病样96份,经分离、纯化、归类出10种分离物,其中镰孢属(Fusarium)真菌占优势。以离体苗干水培接种和盆栽苗接种,经致病性测定,明确分离物Mz01为致病菌株。经形态学鉴定,Mz01为茄镰孢菌(Fusariumsolani)。采用真菌通用引物对分离物Mz01进行PCR扩增,对其核糖体DNA(rDNA)内转录间隔区测序,并将测序结果在GenBank中进行同源性比对分析,分子鉴定与形态学鉴定一致。茄镰孢菌作为我国欧美杨溃疡病的病原菌系首次报道。
A new canker disease occurred on Populus x euramericana cv. ‘74/76＇ and P. x euramerican ‘zhonglin 46＇in Puyang,Henan Province and Heze, Shandong Province in resent years, and caused severe damage to the poplars. Disease incidence increased year after year. Determination of the pathogen of the disease is in an urgent need. Ninety-six samples of the canker were collected from stands of diseased poplars and 10 isolates were obtained, among which most isolates belonged to genus Fusarium. The isolates were inoculated on both hydroponic stem and potted two years old seedlings of Populus × cv. ‘74/76＇ for 4 - 5 days at 25 ℃. Re-isolation from the lesion formed by the inoculation was the same as the inocula, demonstrating that the isolate Mz01 was a pathogenic strain of Fusarium solani based on its morphological. The sequences of the isolates Mz01 were amplyfied by PCR technique with universal primers of fungi, and the sequences were further compared with that in GenBank. The molecular identification confirmed the same results from morphology identification. This is the first report that Fusarium solani is the pathogen of canker disease in Populus × euramericana in China.