文献类型：期刊文献

英文题名：Determination of Community Structure and Diversity of Seed-Vectored Endophytic Fungi in Alpinia zerumbet

作者：Yan, Kuan[1,2] Pei, Zihao[1,2] Meng, Lina[1,2] Zheng, Yu[1,2] Wang, Lian[1,2] Feng, Ruizhang[1,2] Li, Quanzi[3] Liu, Yang[4] Zhao, Xianming[1] Wei, Qin[1,2] El-Sappah, Ahmed H. H.[1,2,5] Abbas, Manzar[1,2]

第一作者：Yan, Kuan

通信作者：Wei, Q[1];El-Sappah, AHH[1];Abbas, M[1];Wei, Q[2];El-Sappah, AHH[2];Abbas, M[2];El-Sappah, AHH[3]

机构：[1]Yibin Univ, Fac Agr Forestry & Food Engn, Yibin, Peoples R China;[2]Yibin Univ, Sichuan Oil Cinnamon Engn Technol Res Ctr, Yibin, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[4]Univ Sci & Technol Beijing, Coll Chem & Biol Engn, Beijing, Peoples R China;[5]Zagazig Univ, Fac Agr, Genet Dept, Zagazig, Egypt

年份：2022

卷号：13

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000771018300001)】；

基金：Funding KY acknowledges that this work was co-supported by the Sichuan Provincial Department of Science and Technology Project (Grant No. 2020JDRC0083) and the Scientific Research Project of Yibin University (Grant No. 2018RC09).

语种：英文

外文关键词：Alpinia zerumbet seed; endophytic fungi; high-throughput sequencing; probiotics; community structure

摘要：Endophytic fungi act as seed endosymbiont, thereby playing a very crucial role in the growth and development of seeds. Seed-vectored endophytic fungi establish an everlasting association with seeds and travel from generation to generation. To explore the composition and diversity of endophytic fungi in Alpinia zerumbet seeds, high-throughput Illumina MiSeq sequencing was employed for the following stages: fruit formation period (YSJ1), young fruit period (YSJ2), early mature period (YSJ3), middle mature period (YSJ4), and late mature period (YSJ5). A total of 906,694 sequence reads and 745 operational taxonomic units (OTUs) were obtained and further classified into 8 phyla, 30 classes, 73 orders, 163 families, 302 genera, and 449 species. The highest endophytic fungal diversity was observed at YSJ5. The genera with the highest abundance were Cladosporium, Kodamaea, Hannaella, Mycothermus, Gibberella, Sarocladium, and Neopestalotiopsis. Functional Guild (FUNGuild) analysis revealed that endophytic fungi were undefined saprotroph, plant pathogens, animal pathogen-endophyte-lichen parasite-plant pathogen-wood saprotroph, and soil saprotrophs. Alternaria, Fusarium, Cladosporium, and Sarocladium, which are potential probiotics and can be used as biocontrol agents, were also abundant. This study is part of the Sustainable Development Goals of United Nations Organization (UNO) to "Establish Good Health and Well-Being."