文献类型：期刊文献

英文题名：Transcriptome analysis of immature xylem in the Chinese fir at different developmental phases

作者：Zhang, Yunxing[1,2,3] Han, Xiaojiao[1,2] Sang, Jian[1,2] He, Xuelian[2] Liu, Mingying[1,2] Qiao, Guirong[1,2] Zhuo, Renying[1,2] He, Guiping[2] Hu, Jianjun[1,4]

第一作者：Zhang, Yunxing

通信作者：He, GP[1];Zhuo, RY[2];He, GP[2]|[a0005ead37386c5134079]何贵平;[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China;[3]Henan Polytech Univ, Inst Architectural & Artist Design, Jiaozuo, Henan, Peoples R China;[4]Chinease Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing, Peoples R China

年份：2016

卷号：4

期号：6

外文期刊名：PEERJ

收录：;Scopus(收录号：2-s2.0-84977138577);WOS:【SCI-EXPANDED(收录号:WOS:000378026800003)】；

基金：This work was supported by Major State Basic Research Development Program of China (No. 2012CB114500), National "Twelfth Five-year" Plan for Science & Technology Support Development Program of China (No. 2012BAD01B0201) and the Bamboo/Tree Breeding Project of Zhejiang Province "Twelfth Five-Year" Plan (No. 2012C12908-11). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Transcriptome; Chinese fir; RNA-Seq; Wood formation; Xylem

摘要：Background. Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] is one of the most important native tree species for timber production in southern China. An understanding of overall fast growing stage, stem growth stage and senescence stage cambium transcriptome variation is lacking. We used transcriptome sequencing to identify the repertoire of genes expressed during development of xylem tissue in Chinese fir, aiming to delineate the molecular mechanisms of wood formation. Results. We carried out transcriptome sequencing at three different cultivation ages (7Y, 15Y and 21Y) generating 68.71 million reads (13.88 Gbp). A total of 140,486 unigenes with a mean size of 568,64 base pairs (bp) were obtained via de novo assembly. Of these, 27,427 unigenes (19.52%) were further annotated by comparison to public protein databases, A total of 5,331 (3.79%) unigenes were mapped into 118 pathways by searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG). Differentially expressed genes (DEG) analysis identified 3, 16 and 5,899 DEGs from the comparison of 7Y vs. 15Y, 7Y vs. 21Y and 15Y vs, 21Y, respectively, in the immature xylem tissues, including 2,638 significantly up-regulated and 3,280 significantly down-regulated genes. Besides, five NAC transcription factors, 190 MYB transcription factors, and 34 WRKY transcription factors were identified respectively from Chinese fir transcriptome. Conclusion. Our results revealed the active transcriptional pathways and identified the DEGs at different cultivation phases of Chinese fir wood formation. This transcriptome dataset will aid in understanding and carrying out future studies on the molecular basis of Chinese fir wood formation and contribute to future artificial production and applications,