文献类型：期刊文献

英文题名：Genome scans for divergent selection in natural populations of the widespread hardwood species Eucalyptus grandis (Myrtaceae) using microsatellites

作者：Song, Zhijiao[1,2,3] Zhang, Miaomiao[2,4] Li, Fagen[2] Weng, Qijie[2] Zhou, Chanpin[2] Li, Mei[2] Li, Jie[2] Huang, Huanhua[5] Mo, Xiaoyong[4] Gan, Siming[1,2]

第一作者：Song, Zhijiao

通信作者：Gan, SM[1];Gan, SM[2];Mo, XY[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Forestry Adm Trop Forestry Res, Key Lab, Guangzhou 510520, Guangdong, Peoples R China;[3]Baoshan Univ, Yuanzheng Rd, Baoshan 678000, Peoples R China;[4]South China Agr Univ, Coll Forestry, 284 Block,Wushan St, Guangzhou 510642, Guangdong, Peoples R China;[5]Guangdong Acad Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000385351900001)】；

基金：We thank Yonghuai Huang for assistance with leaf sampling, Xiaoli Yu for help in the laboratory and Yangkun Li for assistance with data analyses. We also thank Jiangming Yao (Guangxi University) for downloading the climatic data and Jianzhong Luo (China Eucalypt Research Centre) for providing the QST data as well as Yanlong Jia and Liang Kou (CAS) for production of Figure 1. We gratefully acknowledge the valuable comments and suggestions of J. F. Scheepens and an anonymous reviewer that helped to improve our manuscript. This work was financially supported by the Ministry of Science and Technology of China (2013AA102705), the National Natural Science Foundation of China (31270702) and Guangdong Provincial Department of Science and Technology (2014B020202013).

语种：英文

摘要：Identification of loci or genes under natural selection is important for both understanding the genetic basis of local adaptation and practical applications, and genome scans provide a powerful means for such identification purposes. In this study, genome-wide simple sequence repeats markers (SSRs) were used to scan for molecular footprints of divergent selection in Eucalyptus grandis, a hardwood species occurring widely in costal areas from 32 degrees S to 16 degrees S in Australia. High population diversity levels and weak population structure were detected with putatively neutral genomic SSRs. Using three F-ST outlier detection methods, a total of 58 outlying SSRs were collectively identified as loci under divergent selection against three non-correlated climatic variables, namely, mean annual temperature, isothermality and annual precipitation. Using a spatial analysis method, nine significant associations were revealed between F-ST outlier allele frequencies and climatic variables, involving seven alleles from five SSR loci. Of the five significant SSRs, two (EUCeSSR1044 and Embra394) contained alleles of putative genes with known functional importance for response to climatic factors. Our study presents critical information on the population diversity and structure of the important woody species E. grandis and provides insight into the adaptive responses of perennial trees to climatic variations.