文献类型：期刊文献

英文题名：Regeneration Capability Comparison of Leaves Between Nodal Cuttings from Young Stems and Suckers and Its Histological Analysis in Triadica sebifera

作者：Chen, Yuan[1,2] Xie, Yumei[1,2,3] Zheng, Keyuan[1,2] Fan, Yanru[4] Zhou, Huijing[1,2] Zhu, Mulan[1,2]

第一作者：Chen, Yuan

通信作者：Zhu, ML[1];Zhu, ML[2]

机构：[1]Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai 201602, Peoples R China;[2]Chinese Acad Sci, Natl Key Lab Plant Mol Genet NKLPMG, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China;[3]Shanghai Normal Univ, Sch Life Sci, Shanghai 200223, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2025

卷号：16

期号：6

外文期刊名：FORESTS

收录：;EI(收录号：20252618679928);Scopus(收录号：2-s2.0-105009062798);WOS:【SCI-EXPANDED(收录号:WOS:001515618500001)】；

基金：This work was supported by National Key Research and Development Program of China (2023YFD2200604) and Special Fund for Scientific Research of Building National Botanical Garden (XM04-10).

语种：英文

外文关键词：Triadica sebifera; leaf explants; sprouts; direct organogenesis; adventitious shoot; regeneration capacity; in vitro regeneration; morphogenesis

摘要：Triadica sebifera, an economically and medicinally valuable tree species native to China, was investigated for its in vitro regeneration potential using leaf explants from nodal cuttings of young stems and sprouts. This study evaluated the effects of basal media, plant growth regulators (PGRs), explant sources, and incision methods on adventitious shoot induction, supplemented by histological analysis. The highest shoot regeneration frequency (98.89%) and maximum shoot number (72) were achieved via direct organogenesis using sucker-derived nodal cuttings cultured on MS medium with 2 mg/L 6- benzyladenine (6-BA), 0.3 mg/L kinetin (KT), and 0.2 mg/L alpha-naphthaleneacetic acid (NAA). Under identical conditions, branch-derived explants showed lower regeneration (84.44%, 64 shoots). Transverse midvein incision proved most effective, with sucker-derived leaves exhibiting superior regeneration. Shoots elongated completely (100%) on Murashige and Skoog (MS) medium containing 0.3 mg/L 6-BA, 0.03 mg/L NAA, and activated charcoal. Rooting was optimal on MS medium with 0.3 mg/L indole-3-butyric acid (IBA), yielding a 98% acclimatization survival rate. Histological analysis revealed de novo meristem formation from parenchyma cells, confirming direct organogenesis without callus intermediation, further validating the enhanced regenerative capacity of sprout-derived explants. This efficient in vitro regeneration system provides a foundation for large-scale propagation and germplasm conservation of T. sebifera, while offering insights for woody plant regeneration studies.