文献类型：期刊文献

英文题名：Comprehensive analysis of calcium-dependent protein kinase genes reveals the potential role of PeCDPK32 in lignin biosynthesis of Phyllostachys edulis

作者：Li, Mengyun[1,2] Li, Yanan[1] Jia, Yuhan[1] Ouyang, Longfei[1] Xu, Jing[1] Chen, Yu[3] Hua, Keda[4] Sun, Jiaojiao[4] Zhuo, Renying[1] Qiao, Guirong[1]

第一作者：Li, Mengyun

通信作者：Qiao, GR[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Zhejiang Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing 210037, Peoples R China;[3]Agr Technol Extens Ctr Dongtai, Yancheng 224200, Peoples R China;[4]Fuyang Dist Agr & Forestry Resource Conservat Ctr, Hangzhou 311400, Peoples R China

年份：2025

卷号：234

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20253118905177);Scopus(收录号：2-s2.0-105011988269);WOS:【SCI-EXPANDED(收录号:WOS:001544466800001)】；

基金：This work was supported by the National Key Research Development Program of China (Grant No. 2021YFD2200504) and the National Non-profit Institute Research Grant of Chinese Academy of Forestry (Grant No. CAFYBB2020ZB004) .

语种：英文

外文关键词：Calcium-dependent protein kinase; Phyllostachys edulis; Expression pattern; Lignin biosynthesis; Secondary cell wall

摘要：Calcium-dependent protein kinases (CDPKs/CPKs) represent a specialized class of Ca2* sensor proteins that decode intracellular calcium fluctuations and translate these signals into phosphorylation cascades, ultimately modulating downstream gene expression. Despite their established roles in model plant species, the functional characterization of CDPKs in moso bamboo (Phyllostachys edulis) - particularly their involvement in the lignification process of this economically important non-timber forest species - remains largely unexplored. In this study, we identified 50 PeCDPK genes through genome-wide analysis and conducted comprehensive characterization of their phylogenetic relationships, conserved domain architectures, gene structure organization, syntenic conservation patterns, and cis-regulatory element composition. Expression profiling demonstrated both tissue-specific patterns and a striking correlation between PeCDPK32 expression and lignification progression in developing bamboo shoots, as confirmed by integrated transcriptomic and qRT-PCR analyses. Through yeast two-hybrid screening, 50 proteins interacting with PeCDPK32 were identified, including the calmodulin-binding protein PeCML4 and the lignin-biosynthetic peroxidase PePRX2. Subcellular localization analysis demonstrated that both PeCDPK32 and PePRX2 localized to the endoplasmic reticulum (ER). Transient overexpression of PeCDPK32 in Nicotiana benthamiana significantly enhanced PePRX2 enzymatic activity, while stable transformation in P. edulis calli promoted lignin biosynthesis. Two autophosphorylation sites on PeCDPK32 were identified by IP-MS analysis. This study presents a systematic genomic and functional characterization of the CDPK gene family in P. edulis, identifying PeCDPK32 as a central regulator of lignin biosynthesis that modulates the lignification pathway through its interaction with key enzymes in the lignification pathway.